Accelerated gain in fat mass (FM) in early life increases the risk for adult diseases. Longitudinal data on infant body composition are crucial for clinical and research use, but very difficult to obtain due to limited measurement tools and unsuccessful measurements between age 6–24 months. We compared FM% by dual-energy X-ray absorptiometry (DXA), with cushion to reduce movement artifacts, with FM% by air-displacement plethysmography (ADP) and evaluated the reliability of this cushion during DXA by comparing FM% with and without cushion. Subsequently, we constructed sex-specific longitudinal body composition charts from 1–24 months.
In 692 healthy, term-born infants (Sophia Pluto Cohort), FM% was measured by ADP from 1–6 months and DXA with cushion from 6–24 months. At 6 months, FM% was measured in triplicate by ADP and DXA with and without cushion(n = 278), later on in smaller numbers.
At 6 months, mean FM% by DXA with cushion was 24.1 and by ADP 25.0, mean difference of 0.9% (Bland–Altman p = 0.321, no proportional bias). Mean FM% by DXA without cushion was 12.5% higher compared to ADP (Bland–Altman p < 0.001). DXA without cushion showed higher mean FM% compared to DXA with cushion (+11.6%, p < 0.001) at 6 months. Longitudinally, FM% increased between 1–6 months and decreased from 6–24 months(both p < 0.001).
In infants, DXA scan with cushion limits movement artifacts and shows reliable FM%, comparable to ADP. This allowed us to construct longitudinal body composition charts until 24 months. Our study shows that FM% increases from 1–6 months and gradually declines until 24 months.
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We thank all infants and their parents for participating in the Sophia Pluto Study. Furthermore, we greatly acknowledge Mrs. J. van Nieuwkasteele, Mrs. M. Huibregtse-Schouten, Mrs. C. Bruinings-Vroombout, Mrs. E. Lems, Ms. N. Khieroe, Mrs. S. Besteman-Voortman, Mrs. J. Bontenbal-van de Wege, research nurses, for their assistance with data collection.
A.C.S.H.-K. received an independent research grant by Danone Nutricia Research.
Conflict of interest
The Sophia Pluto Study is an investigator-initiated study. D.A. is an employee of Danone Nutricia Research.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Online Trial Registry: NTR, NL7833.
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de Fluiter, K.S., van Beijsterveldt, I.A., Goedegebuure, W.J. et al. Longitudinal body composition assessment in healthy term-born infants until 2 years of age using ADP and DXA with vacuum cushion. Eur J Clin Nutr (2020). https://doi.org/10.1038/s41430-020-0578-7