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Dual-energy X-ray absorptiometry visceral adipose tissue estimates: reproducibility and impact of pre-assessment diet


We examined the reproducibility of dual-energy x-ray absorptiometry (DXA) visceral adipose tissue (VAT) estimates as well as the impact of pre-assessment diet. In a counterbalanced design, 41 adults received 6 DXA scans, while consuming standardized high- and low-carbohydrate (LC) diets. The impact of pre-assessment diet was examined via repeated-measures analysis of variance and reproducibility of VAT estimates was evaluated by technical error of measurement (TEM) and s.e. of a single determination (SESD). VAT area, mass and volume were acutely decreased by ~ 6.5% after the LC diet (P ≤ 0.001), but not the high-carbohydrate diet (P > 0.3). Differences persisted after an overnight fast. TEMs for VAT area, mass and volume were < 5 cm2, ≤ 0.2 kg and < 25 cm3. SESDs for all VAT variables were ≤ 5.5%. In conclusion, DXA VAT estimates exhibit good reproducibility in controlled conditions, but may be impacted by pre-assessment diet.

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Fig. 1


  1. 1.

    Despres JP. Is visceral obesity the cause of the metabolic syndrome? Ann Med 2006;38:52–63.

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Cornier MA, Despres JP, Davis N, Grossniklaus DA, Klein S, Lamarche B, et al. Assessing adiposity: a scientific statement from the American Heart Association. Circulation 2011;124:1996–2019.

    Article  PubMed  Google Scholar 

  3. 3.

    Rothney MP, Xia Y, Wacker WK, Martin F-P, Beaumont M, Rezzi S, et al. Precision of a new tool to measure visceral adipose tissue (VAT) using dual-energy X-Ray absorptiometry (DXA). Obesity 2013;21:E134–E136.

    Article  PubMed  Google Scholar 

  4. 4.

    Ergun DL, Rothney MP, Oates MK, Xia Y, Wacker WK, Binkley NC. Visceral adipose tissue quantification using lunar prodigy. J Clin Densitometry 2013;16:75–78.

    Article  Google Scholar 

  5. 5.

    Tinsley GM, Morales E, Forsse JS, Grandjean PW. Impact of acute dietary manipulations on DXA and BIA body composition estimates. Med Sci Sports Exerc 2017;49:823–32.

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Bone JL, Ross ML, Tomcik KA, Jeacocke NA, Hopkins WG, Burke LM. Manipulation of muscle creatine and glycogen changes DXA estimates of body composition. Med Sci Sports Exerc 2016;49:1029–1035.

  7. 7.

    Ulijaszek SJ, Kerr DA. Anthropometric measurement error and the assessment of nutritional status. Br J Nutr 1999;82:165–77.

    CAS  Article  Google Scholar 

  8. 8.

    Staten MA, Totty WG, Kohrt WM. Measurement of fat distribution by magnetic resonance imaging. Invest Radiol 1989;24:345–9.

    CAS  Article  Google Scholar 

  9. 9.

    Katzmarzyk PT, Greenway FL, Heymsfield SB, Bouchard C. Clinical utility and reproducibility of visceral adipose tissue measurements derived from dual-energy X-ray absorptiometry in White and African American adults. Obesity (Silver Spring) 2013;21:2221–4.

    Article  Google Scholar 

  10. 10.

    Thaete FL, Colberg SR, Burke T, Kelley DE. Reproducibility of computed tomography measurement of visceral adipose tissue area. Int J Obes Relat Metab Disord 1995;19:464–7.

    CAS  PubMed  Google Scholar 

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The original data collection was supported by Baylor University and the present analysis was supported by Texas Tech University.

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Correspondence to Grant M Tinsley.

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The authors declare that they have no competing interests.

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Tinsley, G.M., Forsse, J.S., Morales, E. et al. Dual-energy X-ray absorptiometry visceral adipose tissue estimates: reproducibility and impact of pre-assessment diet. Eur J Clin Nutr 72, 609–612 (2018).

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