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Anti-microbial efficacy of l-glutaminase (EC 3.5.1.2) against multidrug-resistant Pseudomonas aeruginosa infection

Abstract

The aims of this study were isolation-purification and characterization of l-glutaminase from L. gasseri BRLHM clinical isolates and investigation of its efficiency as an antimicrobial agent against multidrug-resistant P. aeruginosa. The MICs of l-glutaminase and gentamicin reference were evaluated by the well-diffusion method. The biofilm on the IUD contraceptive was visualized using atomic force microscopy (AFM) image analyses. The purified l-glutaminase possessed significant antimicrobial activity against P. aeruginosa isolates (p < 0.05), and the antibiofilm formation activity of the purified l-glutaminase was stronger than the antibiofilm activity of the referral standard drug, gentamicin (P < 0.05), which were checked by the inhibition of the biofilm formation on the IUD contraceptive device. Investigations indicated that l-glutaminase may have a crucial role in future clinical applications.

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Acknowledgements

The authors thank the Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq; and the Head and staff members of the Division of Biotechnology, Department of Applied Sciences, University of Technology, and Dean College of Pharmacy, and Head Department of Medicinal Chemistry, College of Pharmacy, Qassim University, Saudi Arabia for support and encouragement during the course of the study.

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Correspondence to Ghassan M. Sulaiman, Hamdoon A. Mohammed or Riaz Khan.

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Mahdi, L.H., Hasoon, B.A., Sulaiman, G.M. et al. Anti-microbial efficacy of l-glutaminase (EC 3.5.1.2) against multidrug-resistant Pseudomonas aeruginosa infection. J Antibiot 77, 111–119 (2024). https://doi.org/10.1038/s41429-023-00678-z

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