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Emblestatin: a new peptide antibiotic from Embleya scabrispora K20-0267

The Journal of Antibiotics volume 76, pages 592–597 (2023)Cite this article

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Abstract

A new peptide, emblestatin (1), was discovered from a culture broth of Embleya scabrispora K20-0267. This strain was isolated from soil using an agar medium containing lysozyme. Based on NMR and mass spectrometric analyses, 1 consists of 2-(2-hydroxyphenyl)-2-oxazoline, β-alanine, glutamine, Nα-methyl-Nω-hydroxyornithine and 3-amino-1-hydroxy-2-piperidone moieties. Further analysis using the advanced Marfey’s method revealed that all amino acids with the stereogenic α-carbon in 1 had the l configuration. Compound 1 exhibited iron chelating activity and weak antibacterial activity against Proteus vulgaris and Staphylococcus aureus.

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Fig. 1
Scheme 1
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Acknowledgements

We are grateful to Distinguished Emeritus Professor Satoshi Ōmura (Kitasato University) for his helpful support and valuable suggestions. This study was partially supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science, and Technology of Japan under Grant Number 19H05685 and the Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from the Japan Agency for Medical Research & Development (AMED) under Grant Numbers JP19am0101096 (Phase I) and JP22ama121035 (Phase II).

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Authors and Affiliations

  1. Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan

    Yuta Awano, Hayama Tsutsumi, Yuta Kikuchi, Aoi Kimishima, Masato Iwatsuki, Yukihiro Asami & Yuki Inahashi

  2. Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo, Japan

    Hayama Tsutsumi, Aoi Kimishima, Masato Iwatsuki, Yukihiro Asami & Yuki Inahashi

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  1. Yuta Awano
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Correspondence to Yuki Inahashi.

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Awano, Y., Tsutsumi, H., Kikuchi, Y. et al. Emblestatin: a new peptide antibiotic from Embleya scabrispora K20-0267. J Antibiot 76, 592–597 (2023). https://doi.org/10.1038/s41429-023-00645-8

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  • DOI: https://doi.org/10.1038/s41429-023-00645-8

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