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Verbalide A~F: new phthalide derivatives from the endophytic fungus Preussia sp. CPCC 400972


There are six new phthalide derivatives Verbalide A ~ F (16) together with another known derivative (7) isolated from the endophytic fungus Preussia sp. CPCC 400972. Their structures were established by comprehensive spectroscopic analyses, including NMR and HRESIMS. In addition, compounds 17 exhibited excellent inhibitory effect against influenza A virus.

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  1. Dunning J, et al. International Sever Acute Respiratory and emerging infection consortium. Antiviral combinations for severe influenza. Lancet Infect Dis. 2014;14:1259–70.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Mills CE, Robins JM, Lipsitch M. Transmissibility of 1918 pandemic influenza. Nature. 2004;432:904–06.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Taubenberger JK, Kash JC, Morens DM. The 1918 influenza pandemic: 100 years of questions answered and unanswered. Sci Transl Med. 2019;11:eaau5485.

    Article  PubMed  Google Scholar 

  4. Sun H, et al. Prevalent Eurasian avian-like H1N1 swine influenza virus with 2009 pandemic viral genes facilitating human infection. Proc Natl Acad Sci USA. 2020;117:17204–10.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Toshiki S, et al. Selecting and using the appropriate influenza vaccine for each individual. Viruses. 2021;13:971.

    Article  Google Scholar 

  6. Michael GI. Antivirals and resistance: influenza virus. Curr Opin Virol. 2011;1:563–73.

    Article  Google Scholar 

  7. Peng XP, et al. New xanthones with antiagricultural fungal pathogen activities from the endophytic fungus Diaporthe goulteri L17. J Agric Food Chem. 2021;69:11216–64.

    Article  CAS  PubMed  Google Scholar 

  8. Strobel G, et al. Natural products from endophytic microorganisms. J Nat Prod. 2004;67:257–68.

    Article  CAS  PubMed  Google Scholar 

  9. Song XQ, et al. Xanthone derivatives from Aspergillus sydowi, an endophytic fungus from the liverwort Scapania ciliata S. Lac and their immunosuppressive activities. Phytochem Lett. 2013;6:318–21.

    Article  CAS  Google Scholar 

  10. Fan Z, et al. A thiopyranchromenone and other chromone derivatives from an endolichenic fungus, Preussia africana. J Nat Prod. 2012;75:230–7.

    Article  Google Scholar 

  11. Paudel B, Bhattarai K, Bhattarai HD. Antimicrobial and antioxidant activities of two polyketides from lichen-endophytic fungus Preussia sp. Z Naturforsch CJ Biosci. 2018;73:161–63.

    Article  CAS  Google Scholar 

  12. Talontsi FM, et al. Antiplasmodial and cytotoxic dibenzofurans from Preussia sp. harboured in Enantia chlorantha Oliv. Fitoterapia. 2014;93:233–8.

    Article  CAS  PubMed  Google Scholar 

  13. Gonzalez M, et al. Biodiversity and chemotaxonomy of Preussia isolates from the Iberian Peninsula. Mycol Prog. 2017;16:1–16.

    Google Scholar 

  14. Du L, et al. Cytotoxic dimeric epipolythiodiketopiperazines from the ascomycetous Fungus Preussia typharum. J Nat Prod. 2014;77:1459–66.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Du L, et al. Diarylcyclopentendione metabolite obtained from a Preussia typharum isolate procured using an unconventional cultivation approach. J Nat Prod. 2012;75:1819–23.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Alejandra L, et al. Phthalides: distribution in nature, chemical reactivity, synthesis, and biological activity. Prog Chem Org Nat Prod. 2017;104:127–246.

    Google Scholar 

  17. Wei XD, et al. Recent advances in natural phthalides: distribution, chemistry, andbiological activities. Fitoterapia. 2022;160:105223.

    Article  CAS  PubMed  Google Scholar 

  18. Yang XL, et al. Phthalide derivatives with antifungal activities against the plant pathogens isolated from the liquid culture of Pestalotiopsis photiniae. J Antibiot. 2011;64:723–27.

    Article  CAS  Google Scholar 

  19. Zhao JY, et al. An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA. Nat Commun. 2022;13:2079.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Gao Q, et al. A cell-based high-throughput approach to identify inhibitors of influenza A virus. Acta Pharm Sin B. 2014;4:301–6.

    Article  PubMed  PubMed Central  Google Scholar 

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This work was financially supported by the National Natural Science Foundation of China (No. 81973220), CAMS Innovation Fund for Medical Sciences (No. 2021-I2M-1-055), the National Microbial Resource Center (No. NMRC-2022-3).

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Correspondence to Wenni He or Liyan Yu.

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Zhang, R., Wang, Y., Cai, G. et al. Verbalide A~F: new phthalide derivatives from the endophytic fungus Preussia sp. CPCC 400972. J Antibiot 76, 613–617 (2023).

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