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A new antibiotic from the culture broth of Dentipellis fragilis

The Journal of Antibiotics volume 76, pages 351–354 (2023)Cite this article

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Abstract

During the search for natural antibiotics from fungal metabolites, a new cyathane diterpenoid, fragilicine A (1), and three known cyathane diterpenoids, erinacines I, A, and B (2–4) were isolated from the culture broth of Dentipellis fragilis. Chemical structures of 1–4 were determined by analyses of 1D- and 2D-NMR and MS data and by comparisons with data of those reported in the literature. These isolated compounds were assessed for their antimicrobial activities against Bacillus subtilis, B. atrophaeus, B. cereus, Listeria monocytogenes, Fusarium oxysporum, Diaporthe sp., and Rhizoctonia solani. These compounds showed weak antimicrobial activities.

Although a unique 5/6/7 tricyclic skeleton, cyathane diterpenoids are familiar natural products in higher fungi, with abundant occurrence in species of the genus Cyathus [1,2,3,4,5,6,7], Hericium [8,9,10,11,12], Sarcodon [13,14,15], Strobilurus [16], Laxitextum [17], and Onychium [18]. This class of natural compounds have been reported to be rich in neurotropic activity [2,3,4, 6, 8,9,10,11, 14, 15]. They also possess anti-inflammatory [1,2,3, 5, 13, 18], anti-malarial [7], cytotoxic [1, 5, 7,8,9, 16], and anti-proliferative [17] activities. In particular, their antimicrobial activities against various pathogenic microorganisms such as Acinetobacter baumannii [7], Aspergillus clavatus [16], Bacillus cereus [7], B. subtilis [17], Candida albicans [7, 16], Enterococcus faecium [7], Escherichia coli [7], Klebsiella pneumoniae [7], Mycobacterium tuberculosis [7], Pseudomonas aeruginosa [16], and Staphylococcus aureus [16, 17] have been reported.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01055657) and authors thank Ms. Ji-Young Oh, Center for University-wide Research Facilities (CURF) at Jeonbuk National University, for performing NMR measurements.

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  1. Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, Korea

    Dae-Won Ki & Bong-Sik Yun

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Correspondence to Dae-Won Ki or Bong-Sik Yun.

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Ki, DW., Yun, BS. A new antibiotic from the culture broth of Dentipellis fragilis. J Antibiot 76, 351–354 (2023). https://doi.org/10.1038/s41429-023-00616-z

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