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Isolation and structure determination of allopteridic acids A–C and allokutzmicin from an unexplored actinomycete of the genus Allokutzneria

The Journal of Antibiotics volume 76, pages 305–315 (2023)Cite this article

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Abstract

Two classes of new polyketides, allopteridic acids A–C (1–3) and allokutzmicin (4), were isolated from the culture extract of an actinomycete of the genus Allokutzneria. The structures of 1–4 were elucidated through the interpretation of NMR and MS analytical data. Compounds 1–3 possess the same carbon skeleton with pteridic acids but their monocyclic core structures are distinct from the spiro-bicyclic acetal structures of pteridic acids. Compound 4 is a linear polyketide of an unprecedented class, featured by a guanidino-terminus and an epoxide modification. Compounds 1–3 promoted the root elongation of germinated lettuce seeds by ca. 10–40% at 1~10 μM whereas 4 retarded the seed growth. Compound 4 exhibited weak antimicrobial activity against Candida albicans with MIC 25 μg mL−1.

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Acknowledgements

P388 cells were obtained from JCRB Cell Bank under an accession code JCRB0017 (Lot. 06252002). This work was supported by JSPS KAKENHI Grant Number 19K05848 to Y. I.

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Authors and Affiliations

  1. Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan

    Chang Liu, Zhiwei Zhang, Keisuke Fukaya, Naoya Oku, Enjuro Harunari, Daisuke Urabe & Yasuhiro Igarashi

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Correspondence to Yasuhiro Igarashi.

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Liu, C., Zhang, Z., Fukaya, K. et al. Isolation and structure determination of allopteridic acids A–C and allokutzmicin from an unexplored actinomycete of the genus Allokutzneria. J Antibiot 76, 305–315 (2023). https://doi.org/10.1038/s41429-023-00611-4

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