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Nocardiopsis changdeensis sp. nov., an endophytic actinomycete isolated from the roots of Eucommia ulmoides Oliv

The Journal of Antibiotics volume 76, pages 191–197 (2023)Cite this article

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Abstract

Strain Mg02T was isolated from roots of Eucommia ulmoides Oliv. collected from Changde City, Hunan Province, China. Strain Mg02T, which exhibited distinct chemotaxonomic characteristics of the genus Nocardiopsis: cell-wall chemotype III/C, i.e., meso-diaminopimelic acid as diagnostic amino acid in whole-cell hydrolysates and menaquinone MK-10 with variable degrees of saturation in the side chain as the predominant isoprenoid quinone, was investigated by a polyphasic approach to determine their taxonomic position. Sequence analysis of the 16S rRNA gene indicated that strain Mg02T is affiliated to the genus Nocardiopsis, having highest sequence similarity to Nocardiopsis flavescens CGMCC 4.5723T (99.1%) and <98.7% to other species of the genus Nocardiopsis with validly published names. Phylogenetic analysis of 16S rRNA gene indicated strain Mg02T formed a separate evolutionary clade, suggesting that it could be a novel Nocardiopsis species. Phylogenomic analysis showed that strain Mg02T was closely related to N. flavescens CGMCC 4.5723T and distinct from the latter according to the clustering patterns. The Average Nucleotide Identity and digital DNA-DNA hybridization values between strain Mg02T and N. flavescens CGMCC 4.5723T were far below the species-level thresholds. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, we think that strain Mg02T should represent a novel Nocardiopsis species, for which the name Nocardiopsis changdeensis sp. nov. is proposed. The type strain is Mg02T (=MCCC 1K06174T = JCM 34709T).

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Funding

This work was supported by Education Department of Hunan Province (21C0515), Natural Science Foundation of Hunan Province (2021JJ50024), Key projects of Hunan Provincial Department of Education (19A340), the Education department of Hunan province in China (21A0418), the Doctoral Start-up project of Hunan University of Arts and Science (21BSQD09) and Innovation Team of Microbial Technology in Hunan University of Arts and Science (202026).

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  1. These authors contributed equally: Ping Mo, Kaiqin Li

Authors and Affiliations

  1. State Key Laboratory of Development Biology of Freshwater Fish Sub-Center for health aquaculture, Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Changde Key Innovation Team for wetland biology and Environmental Ecology, College of life and environmental sciences, Hunan University of Arts and Science, Changde, 415000, Hunan, China

    Ping Mo, Jinhua Zhou, Fumin Zhou, Jiang He & Wansheng Zou

  2. Hunan Institute of Agricultural Biotechnology, Changsha, 410000, Hunan, China

    Kaiqin Li

  3. School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan, 411201, Hunan, China

    Jian Gao

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WZ and JG conceived the idea of the study. PM and KL wrote the main manuscript text. JZ, FZ and JH prepared figures and tables. JH, WZ and JG corrected and reviewed the paper. All authors discussed the results and revised the manuscript.

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Correspondence to Wansheng Zou or Jian Gao.

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Mo, P., Li, K., Zhou, J. et al. Nocardiopsis changdeensis sp. nov., an endophytic actinomycete isolated from the roots of Eucommia ulmoides Oliv. J Antibiot 76, 191–197 (2023). https://doi.org/10.1038/s41429-023-00596-0

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