Four new piericidin rhamnosides (2, 4–6) together with three known piericidins (1, 3, 7) were isolated from the culture broth of the unidentified actinomycete strain TMPU-A0287 as potentiators of antifungal amphotericin B (AmB) activity. The structures of piericidins were elucidated by spectroscopic analyses, including NMR and MS. Compounds 2 and 4–6 possessed a ketone at C-10 and one or two methoxy groups on the rhamnose in their structures. Compounds 1–7 did not exhibit antifungal activity against Candida albicans and all potentiated AmB activity. The MIC values of AmB against C. albicans combined with 1–7 (4.0 μg ml−1) decreased from 0.50 to 0.063 or 0.031 μg ml−1, yielding an 8- or 16-fold increase in AmB activity.
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We express our thanks to Mr. T. Matsuki and S. Sato of Tohoku Medical and Pharmaceutical University for the NMR and mass spectra measurements.
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Yagi, A., Yamaguchi, Y., Kawasaki, K. et al. New piericidin rhamnosides as potentiators of amphotericin B activity against Candida albicans produced by actinomycete strain TMPU-A0287. J Antibiot 76, 65–74 (2023). https://doi.org/10.1038/s41429-022-00581-z