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Geninthiocins E and F, two new cyclic thiopeptides with antiviral activities from soil-derived Streptomyces sp. CPCC 200267 using OSMAC strategy


On the basis of the one strain-many compounds (OSMAC) strategy, two new cyclic thiopeptides, geninthiocins E and F, together with four known geninthiocin derivatives, geninthiocins A, B, C, and val-geninthiocin were isolated from Streptomyces sp. CPCC 200267. Their structures and absolute configurations were elucidated by extensive spectroscopic analyses and Marfey’s method. Geninthiocin E (1), val-geninthiocin (3), geninthiocin A (4), and geninthiocin B (5) exhibited significant anti-influenza A virus activities with the IC50 values of 28.7, 15.3, 7.3, and 18.3 μM, respectively. Compounds 3 and 4 showed moderate antibacterial activities against Staphylococcus aureus.

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The authors thank the mass spectrometry facility of National Center for Protein Sciences at Peking University and Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College for assistance with NMR spectrometer and Dr. Xiaogang Niu for help with NMR spectroscopic data analyses.


The work was financially supported by the National Natural Science Foundation of China (82073744), CAMS Innovation Fund for Medical Sciences (2021-I2M-1-055), the Non-profit Central Research Institute Fund of CAMS (2021-PT350-001), and the National Microbial Resource Center (No. NMRC-2022-3).

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Correspondence to Liyan Yu or Dewu Zhang.

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Fang, Y., Wang, J., Tang, Y. et al. Geninthiocins E and F, two new cyclic thiopeptides with antiviral activities from soil-derived Streptomyces sp. CPCC 200267 using OSMAC strategy. J Antibiot 76, 101–104 (2023).

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