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Isatropolone/isarubrolone Cm from Streptomyces with biological activity of inducing incomplete autophagy

The Journal of Antibiotics volume 75, pages 702–708 (2022)Cite this article

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Abstract

Isatropolones/isarubrolones are Streptomyces secondary metabolites featuring a tropolone ring in the pentacyclic scaffolds of these molecules. They are able to induce complete autophagy in human HepG2 cells. Here, methyl isatropolones (1–2) and isarubrolone (3) are identified from Streptomyces CPCC 204095. They all have a methyl tropolone ring in the pentacyclic scaffold of these molecules resolved by MS and NMR spectra. Biological activity assay indicates that isatropolone Cm (1) and isarubrolone Cm (3) induce incomplete autophagy in human HepG2 cells.

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Acknowledgements

NMR and MS analyses were performed at Nuclear Magnetic Resonance Center of Institute of Materia Medica, CAMS & PUMC. This work was supported by National Key Research and Development Program of China (2018YFA0902000), National Natural Science Foundation of China (81903530), CAMS Innovation Fund for Medical Sciences (CIFMS, 2021-I2M-1-055) and National Microbial Resources center (No. NMRC-2021-3).

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Authors and Affiliations

  1. NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, 100050, Beijing, People’s Republic of China

    Linli Li, Miaoqing Zhang, Shufen Li, Bingya Jiang, Jingpu Zhang, Liyan Yu, Hongyu Liu & Linzhuan Wu

  2. Department of Clinical Pharmacy, The Third Hospital of Hebei Medical University, 050051, Shijiazhuang, People’s Republic of China

    Linli Li

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Correspondence to Shufen Li or Linzhuan Wu.

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41429_2022_575_MOESM1_ESM.doc

Supplementary material Isatropolone/isarubrolone C<sub>m</sub> from <i>Streptomyces</i> with biological activity of inducing incomplete autophagy

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Li, L., Zhang, M., Li, S. et al. Isatropolone/isarubrolone Cm from Streptomyces with biological activity of inducing incomplete autophagy. J Antibiot 75, 702–708 (2022). https://doi.org/10.1038/s41429-022-00575-x

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