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  • Brief Communication
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Cytochalasins from coastal saline soil-derived fungus Aspergillus flavipes RD-13 and their cytotoxicities


Chemical investigation of coastal saline soil-derived fungus Aspergillus flavipes RD-13 led to the isolation of two new seco-cytochalasins (1) and (2) along with nine known analogs. Their structures were elucidated by comprehensive spectral analysis, and the absolute configurations of these two new ones were determined through Rh2(OCOCF3)4-induced CD experiment and chemical interconversions. Moreover, the absolute configuration of a known compound named cytochalasins Z18 (3) was also determined for the first time. Structurally, compounds 1, 2 and 3 were the open ring derivatives of compounds 5, 8, and 4, respectively. All compounds were evaluated for their cytotoxic activities on A549, H1299 and H520 cells and 4 exhibited the strongest inhibitory activities towards the above cell lines with IC50 values of 0.15, 0.23 and 0.43 μg/mL, respectively. Preliminary structure-activity relationship analysis suggested the importance of macrocyclic ring in cytochalasins to confer cytotoxicity.

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This work was supported by the National Natural Science Foundation of China (81903537, 81903102 and 82173737), Natural Science Foundation of Shandong Province (ZR2018BB024 and ZR2019MC026), Traditional Chinese Medicine Technology Development Foundation of Shandong (2019-0521), Science and Technology Development Foundation of Binzhou (2015ZC0304), and Research Foundation of Binzhou Medical University (BY2015KYQD31, BY2017KJ17).

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Correspondence to Guowei Cai or Kaikai Gong.

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Miao, S., Liu, M., Qi, S. et al. Cytochalasins from coastal saline soil-derived fungus Aspergillus flavipes RD-13 and their cytotoxicities. J Antibiot 75, 410–414 (2022).

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