Drug-induced diarrhea is a common adverse drug reaction, especially the one caused by the widespread use of antibiotics. The reduction of probiotics is one reason for intestinal disorders induced by an oral antibiotic. However, the intrinsic mechanism of drug-induced diarrhea is still unknown. In this study, we used metabolomics methods to explore the effects of the classic oral antibiotic, amoxicillin, on the growth and metabolism of Lactobacillus acidophilus, while scanning electron microscopy (SEM) and 3-(4,5-Dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT) assays were employed to evaluate changes in cell activity and morphology. The results showed that cell viability gradually decreased, while the degree of cell wall rupture increased, with increasing amoxicillin concentrations. A non-targeted metabolomics analysis identified 13 potential biomarkers associated with 9 metabolic pathways. The data showed that arginine and proline metabolism, nicotinate and nicotinamide metabolism, pyrimidine metabolism, glycine, serine and threonine metabolism, beta-alanine metabolism, glycerolipid metabolism, tryptophan metabolism, steroid hormone biosynthesis, and histidine metabolism may be involved in the different effects exerted by amoxicillin on L. acidophilus. This study provides potential targets for screening probiotics regulators and lays a theoretical foundation for the elucidation of their mechanisms.
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The datasets analyzed in this manuscript are not publicly available. Requests to access the datasets should be directed to the corresponding author ZS.
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This research was financially supported by the Natural Science Foundation of China, Guangxi Natural Science Foundation, Youth Science Foundation of Guangxi Medical University, the National Training Programs of Innovation and Entrepreneurship for Undergraduates of China, and Guangxi First-class Discipline Project for Pharmaceutical Sciences.
This research was financially supported by the Natural Science Foundation of China (81603533, 81660681, 81360638, and 81560626), Guangxi Natural Science Foundation (2018GXNSFAA138096), Youth Science Foundation of Guangxi Medical University (GXMUYSF18), the National Training Programs of Innovation and Entrepreneurship for Undergraduates of China (Grant No.201410598010), and Guangxi First-class Discipline Project for Pharmaceutical Sciences (No.GXFCDP-PS-2018).
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Guo, Y., Liu, X., Huang, H. et al. Metabolic response of Lactobacillus acidophilus exposed to amoxicillin. J Antibiot 75, 268–281 (2022). https://doi.org/10.1038/s41429-022-00518-6