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Neocurromycin A, a new GRP78 downregulator from Streptomyces sp. RAI364

The Journal of Antibiotics volume 73, pages 790–793 (2020)Cite this article

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Abstract

A new curromycin-related compound, neocurromycin A (2), was isolated from the fermented broth of Streptomyces sp. RAI364. The molecular formula of 2 was established as C35H44N4O7 by ESI–MS and the structure was elucidated by NMR spectroscopic analyses. Neocurromycin A showed selective cytotoxicity against MKN45 human gastric cancer cells in a nutrient-deprived medium with an IC50 of 380 nM and inhibited the expression of the molecular chaperone GRP78 in HT1080 G-L cells in the presence of 10 mM of 2-deoxyglucose with an IC50 of 1.7 µM.

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Acknowledgements

We are grateful to Ms. F. Hasegawa and Ms. N. Sawabe for assistance with mass spectrometry and NMR spectroscopy.

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Authors and Affiliations

  1. Department of Medicinal and Life Science, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan

    Yoichi Hayakawa, Ryodai Yaguchi, Minami Akimoto & Shoko Kimata

  2. National Institute of Advanced Industrial Science and Technology, 2-4-7 Aomi, Koto-ku, Tokyo, 135-0064, Japan

    Kazuo Shin-ya

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  1. Yoichi Hayakawa
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  2. Ryodai Yaguchi
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Correspondence to Yoichi Hayakawa.

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Hayakawa, Y., Yaguchi, R., Akimoto, M. et al. Neocurromycin A, a new GRP78 downregulator from Streptomyces sp. RAI364. J Antibiot 73, 790–793 (2020). https://doi.org/10.1038/s41429-020-0339-9

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