Three new carboxylic acid derivatives, pestallic acids F and G (1 and 2), pestalotiopyrone N (3), and a new diphenylketone derivative named neopestalone (5) were obtained from the liquid cultures of marine alga-derived endophytic fungus Pestalotiopsis neglecta SCSIO41403, along with six known compounds (4, 6–10). The structures of those new compounds were elucidated mainly by analysis of their NMR and MS data. The isolated compounds were evaluated for their anti-Dengue virus and COX-2 inhibitory activities, and two diphenylketone derivatives (5 and 6) exhibited obvious COX-2 inhibitory activities, with the IC50 values being 5.8 and 3.4 μM, respectively.
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This work was supported by grants from the Drug Innovation Major Project of China (2018ZX09735001-002003), National Natural Science Foundation of China (81973235, 21772210, 21977102), and Guangdong Basic and Applied Basic Research Foundation (2019B151502042 and 2018A0303130219) and National Key Research and Development Program of China (2019YFC0312503). We are grateful to the analytical facilities (Z. Xiao, A. Sun, Y. Zhang) in SCSIO.
Conflict of interest
The authors declare that they have no conflict of interest.
Dedicated to Professor William Fenical in recognition of his contributions to marine natural products discovery and drug development.
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Wang, J., Peng, Q., Yao, X. et al. New pestallic acids and diphenylketone derivatives from the marine alga-derived endophytic fungus Pestalotiopsis neglecta SCSIO41403. J Antibiot 73, 585–588 (2020). https://doi.org/10.1038/s41429-020-0308-3