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Bioactive secondary metabolites from the deep-sea derived fungus Aspergillus sp. SCSIO 41029

The Journal of Antibiotics volume 74, pages 156–159 (2021)Cite this article

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Abstract

Two new compounds classified as one new lumazine peptide, penilumamide K (1) and one new sesquiterpene (2), were obtained from the deep-sea derived fungus Aspergillus sp. SCSIO 41029, together with eleven known compounds (3−13). The structures of 1−13 including absolute configurations were determined by detailed NMR spectroscopy, HR-ESI-MS, chemical derivatization, and optical rotation data. Among them, compound 1 represents the first lumazine peptide reported from deep-sea derived fungus. The bioactive assay exhibited that compounds 1, 3, 4, 5, 7 and 10 had significant potency against α-glucosidase with IC50 values ranging from 18.61 to 109.06 μΜ. In addition, compounds 4 and 9 showed strong antibacterial activity against Staphylococcus aureus with MIC values of 0.78 and 6.25 μg ml−1, respectively.

The strain SCSIO 41029 was inoculated in the seed medium (consisting of malt extract 15 g, artificial sea salt 10 g, H2O 1 l, pH 7.4–7.8) in 500-ml Erlenmeyer flasks (200 ml/flask) at 25 °C for 3 days on a rotating shaker (180 rpm). A large-scale fermentation was incubated with 10% seed medium at 25 °C in 1 l × 40 conical flasks with solid rice medium (each flask contained 150 g of rice, 4.5 g of artificial sea salt, and 150 ml H2O). After 32 days, the rice medium was extracted repeatedly with acetone (400 ml/flask). The acetone was evaporated under reduced pressure to obtain an aqueous solution, which was extracted with EtOAc three times. Then the EtOAc solution was concentrated under reduced pressure to afford a crude extract, which was further purified by the column chromatography to obtain compounds 1–13.

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Acknowledgements

This work was financially supported by National Key Research and Development Program of China (2019YFC0312503), Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0406), the National Natural Science Foundation of China (Nos. 21772210 and 41776169), Guangdong MEPP Fund (No. GDOE [2019]A28 and [2020]037), Pearl River S&T Nova Program of Guangzhou (No. 201710010136). We are grateful to Zhihui Xiao, Aijun Sun, Xiaohong Zheng, Yun Zhang, and Xuan Ma in the analytical facility at SCSIO for recording spectroscopic data.

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  1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Weihao Chen, Chunmei Chen, Jieyi Long, Xiuping Lin, Shengrong Liao, Bin Yang, Xuefeng Zhou, Junfeng Wang & Yonghong Liu

  2. University of Chinese Academy of Sciences, 19 Yuquan Road, 100049, Beijing, China

    Weihao Chen, Chunmei Chen, Jieyi Long & Yonghong Liu

  3. Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Sujuan Lan

  4. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458, China

    Xiuping Lin, Shengrong Liao, Bin Yang, Xuefeng Zhou, Junfeng Wang & Yonghong Liu

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  1. Weihao Chen
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Chen, W., Chen, C., Long, J. et al. Bioactive secondary metabolites from the deep-sea derived fungus Aspergillus sp. SCSIO 41029. J Antibiot 74, 156–159 (2021). https://doi.org/10.1038/s41429-020-00378-y

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