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Bactericidal effect of pyridine-2-thiol 1-oxide sodium salt and its complex with iron against resistant clinical isolates of Mycobacterium tuberculosis

The Journal of Antibiotics volume 73, pages 120–124 (2020)Cite this article

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Abstract

The objective of this study was to determine the activity of pyridine-2-thiol 1-oxide sodium salt (Na mpo) and its complex with iron [Fe(mpo)3] against Mycobacterium tuberculosis. The compounds were tested against a standard strain of M. tuberculosis H37Rv (ATCC 27294), with minimal inhibitory concentrations (MIC90) of 7.20 and 1.07 μM to Na mpo and [Fe(mpo)3], respectively, and against three clinical isolates with different genotypic profiles, with MIC values ranging from 0.74 to 6.52 and 0.30 to 2.25 μM to Na mpo and [Fe(mpo)3], respectively. [Fe(mpo)3] was more effective against susceptible strains but both compounds were effective in inhibiting MDR and XDR-TB clinical strains. The profile activity was determined through the methodology of a time-kill curve against standard and clinical strains of M. tuberculosis. Time-kill studies indicated that Na mpo had an early bactericidal activity against H37Rv and clinical isolates, with sterilizing effects observed in 5 and 7 days, respectively, at its MIC90. The anti MDR and XDR-M. tuberculosis activity and bactericidal effect of Na mpo and [Fe(mpo)3] demonstrate their potential as new compounds for the treatment of tuberculosis.

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Acknowledgements

This study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP grants: 2013/14957-5 and 2018/00163-0).

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Authors and Affiliations

  1. Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, SP, Brazil

    Debora L. Campos, Camila M. Ribeiro & Fernando R. Pavan

  2. Área Química Analítica, Facultad de Química, Universidad de la República, Montevideo, Uruguay

    Ignacio Machado

  3. Área Química Inorgánica, Facultad de Química, Universidad de la República, Montevideo, Uruguay

    Dinorah Gambino

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  1. Debora L. Campos
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  4. Dinorah Gambino
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  5. Fernando R. Pavan
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Correspondence to Fernando R. Pavan.

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Campos, D.L., Machado, I., Ribeiro, C.M. et al. Bactericidal effect of pyridine-2-thiol 1-oxide sodium salt and its complex with iron against resistant clinical isolates of Mycobacterium tuberculosis. J Antibiot 73, 120–124 (2020). https://doi.org/10.1038/s41429-019-0243-3

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