Fig. 8 | The Journal of Antibiotics

Fig. 8

From: Targeting adenylate-forming enzymes with designed sulfonyladenosine inhibitors

Fig. 8

Biochemistry of biotin protein ligases. Biotin (8.1) is adenylated to form a biotinyl-AMP intermediate (8.2), which reacts with a lysine side-chain amine nucleophile on the BCCP subunit of acetyl-CoA carboxylase to form an amide product (8.3). In the case of the E. coli enzyme BirA, formation of the adenylate intermediate (8.2) also induces homodimerization of the enzyme, which binds to and represses the bioO biosynthetic operon for biotin. BCCP structure derived from PDB ID: 1BDO [216]. BCCP biotin carboxylate carrier protein

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