Heterologous expression of the fluostatin biosynthetic gene cluster from the marine-derived Micromonospora rosaria SCSIO N160 in Streptomyces albus J1074 led to the isolation of a novel isoindolequinone albumycin (1) and a known isoquinolinequinone mansouramycin A (2). The structure of 1 was elucidated on the basis of detailed 1D and 2D NMR spectroscopic analysis. Mansouramycin A (2) is active against methicillin-resistant Staphylococcus aureus ATCC 43300, with a MIC of 8 μg ml−1, while albumycin (1) displayed negligible antibacterial activities. This study represents another example of activation of secondary metabolites that are non-relevant to the heterologously introduced biosynthetic gene cluster in a bacterial host.
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This work is financially supported in part by the National Natural Science Foundation of China (31820103003, 31700042, 41676165), Guangdong Province (GDME-2018C005, 2015A030308013), and Chinese Academy of Sciences (QYZDJ-SSW-DQC004). We are grateful to Z. Xiao, A. Sun, C. Li, and Y. Zhang in the analytical facilities of SCSIO.
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Huang, C., Yang, C., Zhang, W. et al. Albumycin, a new isoindolequinone from Streptomyces albus J1074 harboring the fluostatin biosynthetic gene cluster. J Antibiot 72, 311–315 (2019). https://doi.org/10.1038/s41429-019-0161-4