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1-Amino-2′-fucosyllactose inhibits biofilm formation by Streptococcus agalactiae

The Journal of Antibiotics (2019) | Download Citation

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Abstract

2′-Fucosyllactose (2′-FL) is a ubiquitous oligosaccharide in human milk. Importantly, this carbohydrate promotes the growth of several strains of Bifidobacteria, a class of beneficial gut commensal, and inhibits epithelial binding of pathogens. In light of these protective effects, we elected to evaluate the potential of 2′-FL to serve as an antibacterial agent against Group B Streptococcus (GBS). While 2′-FL was devoid of any substantial antimicrobial or antibiofilm activity, conversion of 2′-FL to its reducing end β-amine provided a novel antibiofilm compound.

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Acknowledgements

This material is based upon work supported by the National Science Foundation under Grant No. [1847804]. This work was also supported by Vanderbilt University, the Vanderbilt Microbiome Initiative (VMI), the Department of Pediatrics at Vanderbilt University Medical Center, and Prolacta Bioscience for S.D.T. K.M.C. was supported by the Vanderbilt Chemical Biology Interface (CBI) training program (T32 GM065086), the Vanderbilt Pre3 Initiative (travel grant), and the Mitchum E. Warren, Jr. Graduate Research Fellowship. Prof. Jennifer Gaddy is acknowledged for use of her facilities. Prof. Shannon Manning at Michigan State University is acknowledged for generously providing clinical strains of GBS (GBS590 and GBS2). Harrison Thomas is acknowledged for his assistance with the viability and biofilm assays. Ryan Doster is acknowledged for discussions regarding experimental design. Jacky Lu is acknowledged for his assistance with bacterial culturing.

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Affiliations

  1. Department of Chemistry, Vanderbilt University, 7330 Stevenson Center, Nashville, TN, 37235, USA

    • Kelly M. Craft
    •  & Steven D. Townsend
  2. Institute of Chemical Biology, Vanderbilt University, 896 Preston Research Building, Nashville, TN, 37232, USA

    • Steven D. Townsend
  3. Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University, Medical Center North A-5302, 1161 21st Ave South, Nashville, TN, 37232, USA

    • Steven D. Townsend

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The authors declare that they have no conflict to declare.

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Correspondence to Steven D. Townsend.

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DOI

https://doi.org/10.1038/s41429-019-0151-6