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Ala-geninthiocin, a new broad spectrum thiopeptide antibiotic, produced by a marine Streptomyces sp. ICN19

The Journal of Antibioticsvolume 72pages99105 (2019) | Download Citation


Bioassay-guided screening of antibacterial compounds from the cultured marine Streptomyces sp. ICN19 provided Ala-geninthiocin (1), along with its known analogs geninthiocin (2) and Val-geninthiocin (3) and the indolocarbazole staurosporine (4). The structure of 1 was determined on the basis of 1D and 2D NMR spectra and ESI-HRMS. The absolute configurations of the amino acid residues were determined by enantioselective GC-MS analysis. Compound 1 exhibited potent activity against Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis, Mycobacterium smegmatis, and Micrococcus luteus, as well as cytotoxicity against A549 human lung carcinoma cells with an IC50 value of 6 nM.

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The support of the Department of Science and Technology, Science and Engineering Research Board (DST-SERB), Govt. of India [F.No. SR/SO/HS-104/2012] to SGPV is gratefully acknowledged. The European Molecular Biology Organization (EMBO) is gratefully acknowledged for the Short term fellowship grant to AMI [ASTF No. 90 – 2016]. We thank Manfred Nimtz for the GC-MS analysis of amino acids. We thank Christel Kakosche, Romy Schade, and Wera Collisi for their excellent technical assistance.

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  1. International Centre for Nanobiotechnology (ICN), Centre for Marine Science and Technology (CMST), Manonmaniam Sundaranar University, Rajakkamangalam-629502, Kanyakumari District, Tamil Nadu, India

    • Appadurai Muthamil Iniyan
    •  & Samuel Gnana Prakash Vincent
  2. Microbial Strain Collection, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstrasse 7, 38124, Braunschweig, Germany

    • Appadurai Muthamil Iniyan
    •  & Joachim Wink
  3. Department Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstrasse 7, 38124, Braunschweig, Germany

    • Enge Sudarman
  4. German Centre for Infection Research Association (DZIF), Partner site Hannover-Braunschweig, Inhoffenstrasse 7, 38124, Braunschweig, Germany

    • Enge Sudarman
    •  & Joachim Wink
  5. Molecular and Nanomedicine Research Unit, Centre for Nanoscience and Nanotechnology (CNSNT), Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Chennai, 600119, TN, India

    • Rajaretinam Rajesh Kannan


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The authors declare that they have no conflict of interest.

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Correspondence to Samuel Gnana Prakash Vincent.

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