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Streptosporangium minutum sp. nov., isolated from garden soil exposed to microwave radiation

The Journal of Antibioticsvolume 71pages564574 (2018) | Download Citation


The actinobacterium, strain M26T, was isolated from garden soil that was pre-treated with microwave radiation. The soil sample was collected in Roodepoort, Gauteng Province, South Africa as part of an antibiotic-screening programme. The isolate produced branched vegetative mycelium with sporangiophores bearing small sporangia ranging from 3 to 6 μm in diameter. Rapid genus identification revealed that the isolate belongs to the genus Streptosporangium. To confirm this result, the strain was subjected to polyphasic taxonomic characterisation. Chemotaxonomic characteristics were as follows: meso-DAP in the peptidoglycan, the whole-cell hydrolysate yielded madurose, predominant menaquinones were MK9 (21%), MK9(H2) (40%), MK9(H4) (31%) and MK9(H6) (3%); the polar lipid profile included an aminolipid, phosphoglycolipids, phosphatidylethanolamine, and phosphatidylmonomethylethanolamine. In addition, the fatty acid profile showed the presence of C16:0 (12.8%), C17:1ω8c (14.2%), and 10-methyl-C17:0 (15.8%). Furthermore, 16S rRNA gene sequence phylogenetic analysis showed that the strain is closely related to members of the genus Streptosporangium, which supports its classification within the family Streptosporangiaceae. Strain M26T exhibited antibiosis against a range of pathogenic bacteria, including, but not limited to Acinetobacter baumannii ATCC 19606T, Enterobacter cloacae subsp. cloacae ATCC BAA-1143, Enterococcus faecalis ATCC 51299 (vancomycin resistant), Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19111, Mycobacterium tuberculosis H37RvT, Pseudomonas aeruginosa ATCC 27853, Salmonella enterica subsp. arizonae ATCC 13314T, and the methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 (MRSA). The name Streptosporangium minutum is proposed with the type strain M26T (=LMG 28850T =NRRL B-65295T).

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Thank you to Di James for DNA sequencing and Miranda Waldron of the Electron Microscope Unit, University of Cape Town (UCT) for help with scanning electron microscopy. Thank you to Hans G. Trüper for assistance with Latin in deriving the specific epithet for strain M26T. The authors also wish to acknowledge the assistance of Dr Kirby-McCullough (University of the Western Cape) in the sequencing of the genome of strain M26T.


Marilize le Roes-Hill held a research grant from the National Research Foundation (NRF) of South Africa (grant number: 90304). Paul Meyers was the recipient of research grants from the Medical Research Council of South Africa, and the University Research Committee (UCT). Any opinion, findings and conclusions or recommendations expressed in this material are those of the authors and therefore the NRF does not accept any liability in regard thereto.

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  1. Biocatalysis and Technical Biology Research Group, Institute of Biomedical and Microbial Biotechnology, Cape Peninsula University of Technology, PO Box 1906, Bellville, 7535, South Africa

    • Marilize Le Roes-Hill
    • , Kim Durrell
    •  & Alaric Prins
  2. Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa

    • Kim Durrell
  3. Institute for Microbial Biotechnology and Metagenomics, University of the Western Cape, Bellville, 7535, South Africa

    • Alaric Prins
  4. Department of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch, 7701, Cape Town, South Africa

    • Paul R. Meyers


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Correspondence to Marilize Le Roes-Hill.

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