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Antisense DNA cocktail therapy using short ß-1,3-glucan/oligonucleotide complexes

Abstract

Developing an appropriate delivery vehicle for antisense oligonucleotides (AS-ODNs) to improve efficiency has been a central issue for translating antisense therapy into practice. We have proposed a macromolecular complex made from AS-ODNs and β-glucan, schizophyllan (SPG) as such a tool. We recently discovered a low molecular weight complex in which only one ODNs can be complexed and denoted it as a “quantized complex”.  This quantized complex can transfer AS-ODNs into cytosol more efficiently than the conventional large molecular weight complexes. Building on this previous work, this paper presents a new idea of delivering two different AS-ODNs (K-ras and YB-1) simultaneously to the same target cell to synergistically suppress the growth of cancer cells.

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Acknowledgements

We gratefully would like to thank Takuya Matsunaga and Motoko Tanaka for technical help. This work was financially supported by JSPS KAKENHI and by JST: Grant-in-Aid for Scientific Research A (20H00668), Grant-in-Aid for Challenging Exploratory Research (20K20449), and Kitakyushu Innovative Human Resource and Regional Development Program (JPMJSP2149).

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YH and KS contributed equally to this work. This manuscript was written through contributions of all authors. All authors have given approval to the last version of the manuscript.

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Correspondence to Hiroto Izumi or Kazuo Sakurai.

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Hata, Y., Sumiya, K., Izumi, H. et al. Antisense DNA cocktail therapy using short ß-1,3-glucan/oligonucleotide complexes. Polym J (2022). https://doi.org/10.1038/s41428-022-00720-1

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