Bioinspired macromolecular templates for crystallographic orientation control of ZnO thin films through zinc hydroxide carbonate

The biomineralization-inspired preparation of inorganic hybrid materials has attracted attention. Here, we report a new approach to the orientation control of zinc oxide (ZnO) thin-film crystals through the preparation of zinc hydroxide carbonate (ZHC) by the macromolecular templates of poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(vinyl alcohol) (PVA). Using 100-nm-thick PHEMA templates, ZHC thin films with the c-axis oriented parallel to the substrate were obtained, while ZHC thin films prepared by 100-nm-thick PVA templates showed perpendicular orientation. After the thermal treatment of ZHC, the crystal orientations of the ZnO thin films were maintained. The effects of the thickness and annealing time for the polymer templates on the morphologies of the ZnO thin films were examined. An approach inspired by biomineralization has allowed us to develop various organic/inorganic hybrid materials with environmental benignity. In the present study, we have achieved the orientation control of zinc hydroxide carbonate (ZHC) thin-film crystals through the chemical structures and morphologies of the templates. After the thermal treatment of the ZHC thin films, the crystal orientations of the resultant zinc oxide (ZnO) thin films are maintained. The effects of the thickness and annealing time for the polymer templates on the formation of ZnO thin films have been examined.

On the other hand, it has been recognized that amorphous minerals play a key role in the formation of biominerals in nature [6,26,27]. In the synthetic system, amorphous CaCO 3 colloids can be stabilized by polymer additives [28][29][30][31][32]. These amorphous colloids serve as a precursor for spontaneous CaCO 3 crystallization in polymer matrices in aqueous solutions, which is similar to the biomineralization phenomena that occur in nature [29,30,33,34].
Our intention here is to tune the crystallographic orientation for zinc hydroxide carbonate (ZHC) and zinc oxide (ZnO) thin films through the biomimetic macromolecular template approach (Fig. 1). Because ZnO has versatile applications in various electro-optic fields, the crystallization control of ZnO has been widely studied [35][36][37][38][39][40]. In particular, the aqueous-solution-mediated approach attracts much attention as a new way of manufacturing for a sustainable society. Biomineralization-inspired synthetic approaches are attractive because of their simple processes and low energy consumption. Previously, we reported the biomineralization-inspired preparation of ZHC and zinc hydroxide thin-film hybrids, which were converted to ZnO [41][42][43]. In this method, the polymer-stabilized amorphous precursors presumably infiltrate into the polymer matrix and induce crystallization. A ZnO thin film with the c-axis aligned perpendicular to the substrate was obtained by using PVA matrices [41]. In general, ZnO thin films are fabricated by widely used deposition methods, such as sputtering, chemical vapor deposition, and pulse vapor deposition. For these ZnO thin films, the c-axis of ZnO exhibited perpendicular alignment to the substrate [40]. There have been few reports on the synthetic methods of ZnO thin films with the c-axis aligned parallel to the substrate [44][45][46].
Here, we report a new macromolecular template method for the control of perpendicular and parallel orientations of thin-film crystals of ZHC and ZnO based on the PHEMA and PVA templates. To understand the template effects, we also fabricated ZnO thin films by varying the thickness and annealing time of the polymer matrices.

Preparation of zinc hydroxide carbonate and zinc oxide thin films
To prepare the polymer matrix, PHEMA and PVA solutions in dimethylsulfoxide were spin-coated on glass substrates. After the matrix was annealed at 200°C for 10 min, it was immersed in the solution prepared by mixing zinc nitrate hexahydrate aqueous solution containing PAA and ammonium carbonate aqueous solution. The final concentration of zinc nitrate hexahydrate and ammonium carbonate was 20 mM, and that of PAA was adjusted to 7.2 × 10 −2 wt%. The PVA matrices were immersed in 4 ml of the solution for 24 h at 40°C. After the crystallization processes, the samples were rinsed with purified water and dried in air. The resultant samples were annealed at 500°C for 2 h.

Characterization
The morphologies of the samples were observed with scanning electron microscopy (SEM) (JSM-7800F Prime, operated at 3.0 kV, JEOL, Tokyo, Japan). SEM observations were performed with osmium coating. The crystal structures of the hybrids were analyzed by X-ray diffraction (XRD) (Smartlab, Rigaku, Tokyo, Japan) measurements using a parallel beam method with CuKα radiation (λ = 0.154 nm). To examine the crystallographic orientation, XRD measurements of the thin-film samples were recorded in two different geometries (i.e., out-of-plane with 2θ scanning and in-plane with 2θχ scanning). The scanning directions of the out-of-plane and in-plane are perpendicular to the substrate and parallel to the substrate, respectively ( Supplementary Fig. S1). The samples collected from the glass substrates were observed with transmission electron microscopy (TEM) (JEM-2000EX, operated at 200 kV, JEOL, Tokyo, Japan). The surfaces of the samples were observed with atomic force microscopy (AFM) (Multi-mode8, Bruker Japan K.K., Kanagawa, Japan) in the air at room temperature. Thermogravimetric measurements

Results and discussion
The PHEMA and PVA thin-film matrices with 100 nm thickness were prepared by spin-coating and subsequent annealing ( Supplementary Fig. S2). After the crystallization process, spherulitic thin-film crystals were observed in the matrices by SEM, and they fully cover the substrates, forming flat thin films (Fig. 2, left and middle). The thickness of the thin-film hybrids was determined by crosssectional SEM images (Fig. 2, right). The thickness of hybrids consisting of ZHC and PHEMA (ZHC/PHEMA) and ZHC/PVA thin-film hybrids was 250 nm. Figure 3 shows XRD patterns of the as-prepared thinfilm crystals. All the peaks observed in the XRD patterns are assigned to ZHC (Zn(CO 3 ) x (OH) y ·nH 2 O; x = 0.25, y = 1.5, n = 0.25 in ICDD no. 011-0287 and x = 0.4, y = 1.2, n = 0 in ICDD no. 019-1458, respectively). The powder sample of the thin-film crystals formed in the PHEMA matrix was collected from the substrate, which also shows a diffraction pattern of ZHC without any distinct orientation ( Supplementary Fig. S3).
Preferential crystallographic orientations of ZHC crystals were observed in the XRD patterns of thin-film samples. In the out-of-plane XRD pattern of thin-film samples grown in the PHEMA matrix, the intensified peak of the (200) plane is seen except for the broad peak of the glass substrate at 2θ =~25°. In contrast, the in-plane XRD pattern exhibits an intensified peak of the (002) plane of the ZHC crystals.
ZHC crystals grown in the PHEMA matrix were preferentially oriented along the (200) plane parallel to the   In the previous studies on CaCO 3 thin film formation, no such drastic change in crystallographic orientation was observed [13][14][15][16][17]. Because ZHC has an anisotropic layered crystal structure [47], the crystal growth direction may easily be affected by the environment. When the bare glass substrate was used in place of the polymer matrix, no crystallization was observed.
These thin-film crystals were converted to ZnO thin films by annealing at 500°C for 2 h (Fig. 4). Both samples maintained their thin-film morphologies after the thermal treatment. The thickness of the ZnO thin film prepared by using the PHEMA matrix was 150 nm, and that of the ZnO thin film prepared by using the PVA matrix was 200 nm.
XRD patterns of the annealed samples show that ZnO was formed (Fig. 5). The intensities of the (002) peaks are different between the out-of-plane and in-plane XRD patterns. This observation suggests that these ZnO thin films had specific c-axis orientations, which were the same as those of the precursor ZHC thin films (Fig. 3). These results indicate that the orientation of ZnO thin films reflects the orientation of ZHC before the conversion. It is noteworthy that the ZnO thin films with the c-axis oriented parallel to the substrate were obtained. This orientation is not easy to achieve by widely used methods [40].
The nanostructures of the ZnO thin films were examined with AFM and TEM. The AFM images (Fig. 6) show that the top surface of both samples had a homogeneous distribution of nanocrystals. The TEM observation was carried out on the samples after scraping the surface of the thin films. The TEM images (Fig. 7) show that both samples were composed of granular crystals with sizes of 10-50 nm, and all the polymer matrices were apparently decomposed.
To examine the relationship between the polymer matrices and oriented crystallization behavior, the effects of the thickness and annealing time for the matrices on the crystal orientation were studied. The PHEMA and PVA matrices with 100, 300, and 1000 nm thickness annealed at 200°C for 10, 30, and 90 min (Supplementary Figs. S4 and S5) were used for ZHC crystallization for 24 h. Figure 8 shows the out-of-plane XRD patterns of the as-prepared ZHC thin films. The orientation of ZHC crystals grown in PHEMA matrices is strongly dependent on the thickness and annealing time. The PHEMA matrices with 100 nm thickness annealed for 10 min induce the ZHC crystals to align along the (200) plane parallel to the substrate. In contrast, the peak attributed to the (002) plane of ZHC was observed for the PHEMA matrices with a thickness of 1000 nm that were annealed for 90 min. For the PVA matrices, the ZHC crystals were oriented along the (002) plane parallel to the substrate, and this orientation was not affected by the thickness and annealing time.
The ZHC thin films grown in polymer matrices were converted to ZnO thin films by annealing at 500°C for 2 h. Figure 9 shows the out-of-plane XRD patterns of ZnO thin films. These results show that the c-axis orientation of the resultant ZnO crystals prepared by PHEMA matrices strongly depends on the thickness and annealing time of the matrices. The ZnO thin films prepared by PHEMA matrices with a thickness of 1000 nm and 90 min annealing indicated an intensified peak of the (002) plane. As the thickness and annealing time for the PHEMA matrices decreased, the intensities of the (002) peak decreased. Using PHEMA matrices with 100 nm thickness and 10 min annealing, the (002) peak disappeared, which suggests that the c-axis is oriented parallel to the substrate. In contrast, PVA matrices induce the c-axis of ZnO crystals to align perpendicular to the substrate almost independently of the thickness and annealing time.
It was reported that the alignment of the plate-like crystals of ZHC depends on the hydrophilicity of the substrate [46]. Taking these results into account, we hypothesized that the hydrophilic environment inside the PHEMA matrices was changed by thermal treatment, while the environment inside the PVA matrices was not changed. In addition to the effects of thermal treatment, the matrix 10 20 30 Fig. 9 Out-of-plane XRD patterns of the ZnO thin films prepared by using a-c PHEMA and d-f PVA matrices with a, d 100 nm, b, e 300 nm, and c, f 1000 nm thickness annealed at 200°C for 10, 30, and 90 min thickness also affects the hydrophilic environment inside the matrix. It is assumed that the effects of thickness for PHEMA matrices on the hydrophilicity were more significant than those of PVA because the PHEMA matrices were more hydrophobic than the PVA matrices. The PHEMA matrices in the vicinity of the water-matrix interface were highly swollen, while the other part of the thicker matrices tended to be relatively more hydrophobic. Therefore, for PHEMA matrices, the hydrophobic part might increase in proportion to the matrix thickness.

Conclusions
In summary, we have achieved control of the crystallographic orientation of ZnO thin films based on ZHC thinfilm hybrids prepared by the biomimetic macromolecular template approach. The PHEMA and PVA matrices largely affected the c-axis orientation of the ZHC thin films. It is noteworthy that ZHC and ZnO thin films prepared by using the PHEMA matrix exhibited drastic changes in the c-axis orientation depending on the thickness and annealing time of the matrices. We demonstrate that not only the preparation of ZnO thin films but also the tuning of the orientation is possible in a wide range with the combination of the biomimetic macromolecular template approach and thermal treatment. We assume that this method is promising for the future development of new ZnO materials with controlled structures. It is also important that biomimetic syntheses of organic/inorganic hybrids are promising for a sustainable society [48][49][50].