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Preparation of a deformable nanocapsule by living radical polymerization in a liposome


We aimed to prepare an elastic nanocapsule by internal modification of a liposome with living radical polymerization. First, monomers and photoiniferters were encapsulated in liposomes. Then, UV light irradiation was used to conduct living radical polymerization in the liposomes. The obtained nanocapsules showed good absorption and retention of chemical substances depending on the binding ability to the incorporated polymers, and the absorption capacity could be controlled by the length and dissolved state of the polymer chains. In addition, dynamic morphological changes in the capsules were observed when liposomes with high membrane fluidity were employed. Morphological changes in the nanocapsules were induced by altering the pH value and ionic strength, resulting in the release of cargo in response to these environmental conditions.

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  1. Bangham AD, Horne RW. Negative staining of phospholipids and their structural modification by surface active agents as observed in electron microscope. J Mol Biol. 1964;8:660–8.

    Article  CAS  Google Scholar 

  2. Moussaoui N, Cansell M, Denziot A. Marinosomes((R)), Marine lipid-based liposomes: physical characterization and potential application in cosmetics. Int J Pharmaceutics. 2002;242:361–5.

    Article  CAS  Google Scholar 

  3. Weiner N, Lieb L, Niemiec S, Ramachandran C, Hu Z, Egbaria K. Liposomes - a novel topical delivery system for pharmaceutical and cosmetic applications. J Drug Target. 1994;2:405–10.

    Article  CAS  Google Scholar 

  4. Lasic DD. Recent developments in medical applications of liposomes: sterically stabilized liposomes in cancer therapy and gene delivery in vivo. J Controlled Release. 1997;48:203–22.

    Article  CAS  Google Scholar 

  5. Ringsdorf H, Schlarb B, Venzmer J. Molecular architecture and function of polymeric oriented systems - models for the study of organization, surface recognition, and dynamics of biomembranes. Angew Chem Int Ed. 1988;27:113–58.

    Article  Google Scholar 

  6. Bronich TK, Solomatin SV, Yaroslavov AA, Eisenberg A, Kabanov VA, Kabanov AV. Steric stabilization of negatively charged liposomes by cationic graft copolymer. Langmuir. 2000;16:4877–81.

    Article  CAS  Google Scholar 

  7. Takeuchi H, Matsui Y, Yamamoto H, Kawashima Y. Mucoadhesive properties of carbopol or chitosan-coated liposomes and their effectiveness in the oral administration of calcitonin to rats. J Controlled Release. 2003;86:235–42.

    Article  CAS  Google Scholar 

  8. Zong W, Hu Y, Su YC, Luo N, Zhang XN, Li QC, et al. Polydopamine-coated liposomes as pH-sensitive anticancer drug carriers. J Microencapsul. 2016;33:257–62.

    Article  CAS  Google Scholar 

  9. Fujimoto K, Toyoda T, Fukui Y. Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome. Macromolecules. 2007;40:5122–8.

    Article  CAS  Google Scholar 

  10. Fukui Y, Fujimoto K. The preparation of sugar polymer-coated nanocapsules by the layer-by-layer deposition on the liposome. Langmuir. 2009;25:10020–5.

    Article  CAS  Google Scholar 

  11. Fukui Y, Otsuka H, Fujimoto K. Controlled release and targeting of polypeptide-deposited liposomes by enzymatic degradation. Polym J. 2019;51:1223–30.

    Article  CAS  Google Scholar 

  12. Okamoto Y, Taguchi K, Yamasaki K, Sakuragi M, Kuroda S, Otagiri M. Albumin-encapsulated liposomes: a novel drug delivery carrier with hydrophobic drugs encapsulated in the inner aqueous core. J Pharm Sci. 2018;107:436–45.

    Article  CAS  Google Scholar 

  13. Torchilin VP, Klibanov AL, Ivanov NN, Ringsdorf H, Schlarb B. Polymerization of liposome-encapsulated hydrophilic monomers. Makromol Chem Rapid Commun. 1987;8:457–60.

    Article  CAS  Google Scholar 

  14. Wang Y, Tu S, Pinchuk AN, Xiong MP. Active drug encapsulation and release kinetics from hydrogel-in-liposome nanoparticles. J Colloid Interface Sci. 2013;406:247–55.

    Article  CAS  Google Scholar 

  15. Dayani Y, Malmstadt N. Liposomes with double-stranded DNA anchoring the bilayer to a hydrogel core. Biomacromolecules. 2013;14:3380–5.

    Article  CAS  Google Scholar 

  16. Bonifacino JS, Glick BS. The mechanisms of vesicle budding and fusion. Cell. 2004;116:153–66.

    Article  CAS  Google Scholar 

  17. Brangwynne CP, Eckmann CR, Courson DS, Rybarska A, Hoege C, Gharakhani J, et al. Germline P granules are liquid droplets that localize by controlled dissolution/condensation. Science. 2009;324:1729–32.

    Article  CAS  Google Scholar 

  18. Devrim YG, Rzaev ZMO, Piskin E. Synthesis and characterization of poly ((maleic anhydride)-alt-styrene)-co-(2-acrylamido-2-methyl-1-propane sulfonic acid). Macromol Chem Phys. 2006;207:111–21.

    Article  CAS  Google Scholar 

  19. Otsu T. Iniferter concept and living radical polymerization. J Polym Sci Part a Polym Chem. 2000;38:2121–36.

    Article  CAS  Google Scholar 

  20. Otsu T, Matsunaga T, Doi T, Matsumoto A. Features of living radical polymerization of vinyl monomers in homogeneous system using N,N-diethyldithiocarbamate derivatives as photoiniferters. Eur Polym J. 1995;31:67–78.

    Article  CAS  Google Scholar 

  21. Antonietti M, Landfester K. Polyreactions in miniemulsions. Prog Polym Sci. 2002;27:689–757.

    Article  CAS  Google Scholar 

  22. Capek I. On inverse miniemulsion polymerization of conventional water-soluble monomers. Adv Colloid Interface Sci. 2010;156:35–61.

    Article  CAS  Google Scholar 

  23. Forster V, Signorell RD, Roveri M, Leroux JC. Liposome-supported peritoneal dialysis for detoxification of drugs and endogenous metabolites. Sci Transl Med. 2014;6:1–9.

  24. Canham PB. Minimum energy of bending as a possible explanation of biconcave shape of human red blood cell. J Theor Biol. 1970;26:61–81.

    Article  CAS  Google Scholar 

  25. Kusters R, Simon C, Dos Santos RL, Caorsi V, Wu SS, Joanny JF, et al. Actin shells control buckling and wrinkling of biomembranes. Soft Matter. 2019;15:9647–53.

    Article  CAS  Google Scholar 

  26. Okano T, Inoue K, Koseki K, Suzuki H. Deformation modes of giant unilamellar vesicles encapsulating biopolymers. ACS Synth Biol. 2018;7:739–47.

    Article  CAS  Google Scholar 

  27. Dash S, Murthy PN, Nath L, Chowdhury P. Kinetic modeling on drug release from controlled drug delivery systems. Acta Poloniae Pharmaceutica. 2010;67:217–23.

    CAS  PubMed  Google Scholar 

  28. Watanabe Y, Aramaki K, Kadomatsu Y, Tanaka K, Konno Y. Preparation of bicelles using the semi-spontaneous method. Chem Lett. 2016;45:558–60.

    Article  CAS  Google Scholar 

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This work was supported in part by financial support from the Iketani Science and Technology Foundation and KOSÉ Cosmetology Research Foundation.

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Correspondence to Yuuka Fukui.

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Fukui, Y., Fukui, A. & Fujimoto, K. Preparation of a deformable nanocapsule by living radical polymerization in a liposome. Polym J 54, 893–901 (2022).

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