We aimed to prepare an elastic nanocapsule by internal modification of a liposome with living radical polymerization. First, monomers and photoiniferters were encapsulated in liposomes. Then, UV light irradiation was used to conduct living radical polymerization in the liposomes. The obtained nanocapsules showed good absorption and retention of chemical substances depending on the binding ability to the incorporated polymers, and the absorption capacity could be controlled by the length and dissolved state of the polymer chains. In addition, dynamic morphological changes in the capsules were observed when liposomes with high membrane fluidity were employed. Morphological changes in the nanocapsules were induced by altering the pH value and ionic strength, resulting in the release of cargo in response to these environmental conditions.
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Bangham AD, Horne RW. Negative staining of phospholipids and their structural modification by surface active agents as observed in electron microscope. J Mol Biol. 1964;8:660–8.
Moussaoui N, Cansell M, Denziot A. Marinosomes((R)), Marine lipid-based liposomes: physical characterization and potential application in cosmetics. Int J Pharmaceutics. 2002;242:361–5.
Weiner N, Lieb L, Niemiec S, Ramachandran C, Hu Z, Egbaria K. Liposomes - a novel topical delivery system for pharmaceutical and cosmetic applications. J Drug Target. 1994;2:405–10.
Lasic DD. Recent developments in medical applications of liposomes: sterically stabilized liposomes in cancer therapy and gene delivery in vivo. J Controlled Release. 1997;48:203–22.
Ringsdorf H, Schlarb B, Venzmer J. Molecular architecture and function of polymeric oriented systems - models for the study of organization, surface recognition, and dynamics of biomembranes. Angew Chem Int Ed. 1988;27:113–58.
Bronich TK, Solomatin SV, Yaroslavov AA, Eisenberg A, Kabanov VA, Kabanov AV. Steric stabilization of negatively charged liposomes by cationic graft copolymer. Langmuir. 2000;16:4877–81.
Takeuchi H, Matsui Y, Yamamoto H, Kawashima Y. Mucoadhesive properties of carbopol or chitosan-coated liposomes and their effectiveness in the oral administration of calcitonin to rats. J Controlled Release. 2003;86:235–42.
Zong W, Hu Y, Su YC, Luo N, Zhang XN, Li QC, et al. Polydopamine-coated liposomes as pH-sensitive anticancer drug carriers. J Microencapsul. 2016;33:257–62.
Fujimoto K, Toyoda T, Fukui Y. Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome. Macromolecules. 2007;40:5122–8.
Fukui Y, Fujimoto K. The preparation of sugar polymer-coated nanocapsules by the layer-by-layer deposition on the liposome. Langmuir. 2009;25:10020–5.
Fukui Y, Otsuka H, Fujimoto K. Controlled release and targeting of polypeptide-deposited liposomes by enzymatic degradation. Polym J. 2019;51:1223–30.
Okamoto Y, Taguchi K, Yamasaki K, Sakuragi M, Kuroda S, Otagiri M. Albumin-encapsulated liposomes: a novel drug delivery carrier with hydrophobic drugs encapsulated in the inner aqueous core. J Pharm Sci. 2018;107:436–45.
Torchilin VP, Klibanov AL, Ivanov NN, Ringsdorf H, Schlarb B. Polymerization of liposome-encapsulated hydrophilic monomers. Makromol Chem Rapid Commun. 1987;8:457–60.
Wang Y, Tu S, Pinchuk AN, Xiong MP. Active drug encapsulation and release kinetics from hydrogel-in-liposome nanoparticles. J Colloid Interface Sci. 2013;406:247–55.
Dayani Y, Malmstadt N. Liposomes with double-stranded DNA anchoring the bilayer to a hydrogel core. Biomacromolecules. 2013;14:3380–5.
Bonifacino JS, Glick BS. The mechanisms of vesicle budding and fusion. Cell. 2004;116:153–66.
Brangwynne CP, Eckmann CR, Courson DS, Rybarska A, Hoege C, Gharakhani J, et al. Germline P granules are liquid droplets that localize by controlled dissolution/condensation. Science. 2009;324:1729–32.
Devrim YG, Rzaev ZMO, Piskin E. Synthesis and characterization of poly ((maleic anhydride)-alt-styrene)-co-(2-acrylamido-2-methyl-1-propane sulfonic acid). Macromol Chem Phys. 2006;207:111–21.
Otsu T. Iniferter concept and living radical polymerization. J Polym Sci Part a Polym Chem. 2000;38:2121–36.
Otsu T, Matsunaga T, Doi T, Matsumoto A. Features of living radical polymerization of vinyl monomers in homogeneous system using N,N-diethyldithiocarbamate derivatives as photoiniferters. Eur Polym J. 1995;31:67–78.
Antonietti M, Landfester K. Polyreactions in miniemulsions. Prog Polym Sci. 2002;27:689–757.
Capek I. On inverse miniemulsion polymerization of conventional water-soluble monomers. Adv Colloid Interface Sci. 2010;156:35–61.
Forster V, Signorell RD, Roveri M, Leroux JC. Liposome-supported peritoneal dialysis for detoxification of drugs and endogenous metabolites. Sci Transl Med. 2014;6:1–9.
Canham PB. Minimum energy of bending as a possible explanation of biconcave shape of human red blood cell. J Theor Biol. 1970;26:61–81.
Kusters R, Simon C, Dos Santos RL, Caorsi V, Wu SS, Joanny JF, et al. Actin shells control buckling and wrinkling of biomembranes. Soft Matter. 2019;15:9647–53.
Okano T, Inoue K, Koseki K, Suzuki H. Deformation modes of giant unilamellar vesicles encapsulating biopolymers. ACS Synth Biol. 2018;7:739–47.
Dash S, Murthy PN, Nath L, Chowdhury P. Kinetic modeling on drug release from controlled drug delivery systems. Acta Poloniae Pharmaceutica. 2010;67:217–23.
Watanabe Y, Aramaki K, Kadomatsu Y, Tanaka K, Konno Y. Preparation of bicelles using the semi-spontaneous method. Chem Lett. 2016;45:558–60.
This work was supported in part by financial support from the Iketani Science and Technology Foundation and KOSÉ Cosmetology Research Foundation.
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Fukui, Y., Fukui, A. & Fujimoto, K. Preparation of a deformable nanocapsule by living radical polymerization in a liposome. Polym J 54, 893–901 (2022). https://doi.org/10.1038/s41428-022-00632-0