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Poly(ethylene glycol)-based biofunctional hydrogels mediated by peroxidase-catalyzed cross-linking reactions

Polymer Journal volume 52, pages 899–911 (2020)Cite this article

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Abstract

Biofunctional hydrogels prepared by a peroxidase, especially horseradish peroxidase (HRP), serve as an excellent class of materials or platform for the development of cellular scaffolds because their biocompatibility and mild and tunable reaction conditions provide them with desirable properties. In this focus review, we summarize our decade of research into HRP-mediated fabrication of biofunctional hydrogels and their applications, in particular cell culture scaffolds. A brief overview of potential substrates employed in HRP and improvement of the HRP hydrogelation system from the initial step until the hydrogen peroxide removal stage in an effort to meet environmental standards is discussed. We highlight our system and describe its biocompatibility and ability to functionalize molecules to support biofabrication by increasing cellular adhesiveness, retaining growth factor affinity, and finally accelerating the formation of two- and three-dimensional multicellular architectures. In the last section, we outline the adoption of hydrogelation as a self-standing, compartmentalized reaction system, i.e., the use of hydrogel marble to conduct cell-free biosynthesis. We believe that this HRP-mediated hydrogel system offers great potential not only as a cell culture scaffold but also for various biomedical applications.

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Acknowledgements

This work was supported in part by the Japan Society for the Promotion of Science (JSPS) KAKENHI No. JP17K19016. The authors thank the Center of Advanced Instrumental Analysis, Kyushu University, and Nanotechnology Platform Program of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) for facility support as well as Dr Kosuke Minamihata for the discussion and assistance in protein production.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan

    Rie Wakabayashi, Wahyu Ramadhan, Masahiro Goto & Noriho Kamiya

  2. Department of Chemical and Biological Engineering, National Institute of Technology, Sasebo College, Nagasaki, Japan

    Kousuke Moriyama

  3. Division of Biotechnology, Center for Future Chemistry, Kyushu University, Fukuoka, Japan

    Masahiro Goto & Noriho Kamiya

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  1. Rie Wakabayashi
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Wakabayashi, R., Ramadhan, W., Moriyama, K. et al. Poly(ethylene glycol)-based biofunctional hydrogels mediated by peroxidase-catalyzed cross-linking reactions. Polym J 52, 899–911 (2020). https://doi.org/10.1038/s41428-020-0344-7

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