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Development of chitinous nanofiber-based flexible composite hydrogels capable of cell adhesion and detachment

Abstract

Chitinous nanofibers (NFs) exhibit a specific physiological activity not observed in the powdered form. While three-dimensional cell culture scaffolds are being actively studied, attempts have been made to use chitinous materials as well. In this study, we aimed to develop a cell-adhesive chitinous NF-based flexible hydrogel. Such a gel will be easy to handle, and there is a possibility that the gel will be able to be three-dimensionally molded. For this purpose, the surface of chitinous NFs was modified to introduce vinyl polymerizable functional groups, and the modified NFs were copolymerized with an N-isopropylacrylamide (NIPAM) monomer in an aqueous system. First, by modification of chitosan NFs with maleic anhydride (MA), a flexible gel was obtained by subsequent copolymerization with NIPAM. However, the adhesion of L929 mouse fibroblasts onto MA-modified NFs was extremely lower than that of chitosan NFs, probably due to the simultaneous introduction of anionic groups. The desired cell-adhesive flexible hydrogel was eventually obtained by introducing cationic groups and polymerizable functional groups on the chitinous NF surface. Cells adhered to the gel could be detached by cooling to 4 °C. The composite hydrogel fabricated here may be useful as a cell culture scaffold material in regenerative medicine.

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Acknowledgements

This work was partially financed by a Grant-in-Aid for Scientific Research (A) (No. 17H01480) from the Japan Society for the Promotion of Science and JST-Mirai Program (JPMJMI18E3) from the Japan Science and Technology Agency (JST).

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Correspondence to Yoshikuni Teramoto.

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Goto, K., Teramoto, Y. Development of chitinous nanofiber-based flexible composite hydrogels capable of cell adhesion and detachment. Polym J 52, 959–967 (2020). https://doi.org/10.1038/s41428-020-0324-y

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