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One-pot amphiphilic engineering of bioresorbable polymers for constructing colloidal vesicles and prolonging protein delivery

Polymer Journal volume 52, pages 237–244 (2020)Cite this article

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Abstract

In this study, we sought to develop methods for amphiphilic engineering of bioresorbable polymers, sorbitan–poly(lactic acid) (sorbitan–PLA) and poly(ethylene glycol)–polylactic acid (PEG–PLA), by melt polycondensation of lactic acid in the presence of the binary initiators sorbitan and methoxy PEG in a single reactor. Briefly, oligo(lactic acid) (OLA) was first prepared by distilling water out of lactic acid under vacuum; then, sorbitan and methoxy PEG were introduced in the reactor, followed by the simultaneous polycondensation of OLA onto sorbitan and methoxy PEG, resulting in a mixture containing sorbitan–PLA/PEG–PLA copolymers. Without further purification, the recovered products were dissolved in saline buffer, mixed with squalane oil, and then homogenized to construct a stable colloidal vesicle. Then, we conducted a mechanistic study to progressively elucidate the relationship between the dispersion structure and the sustained release of a model protein bovine serum albumin. This one-pot approach has potential for applications and commercial use in the field of biodegradable controlled-release delivery systems.

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Acknowledgements

This work was supported by the National Health Research Institutes of Taiwan (grant number 108A1-IVPP19-014) and by a grant from the Ministry of Science and Technology of Taiwan (grant number MOST 106-2314-B-400-016-MY3).

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Author notes

  1. These authors contributed equally: Chiung-Yi Huang, Yu-Jhen Cheng

Authors and Affiliations

  1. National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, 35053, Taiwan

    Chiung-Yi Huang, Yu-Jhen Cheng, Hui-Min Ho & Ming-Hsi Huang

  2. Department of Food Science, National Taiwan Ocean University, Keelung, 20224, Taiwan

    Chung-Hsiung Huang

  3. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, 40402, Taiwan

    Ming-Hsi Huang

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  1. Chiung-Yi Huang
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  2. Yu-Jhen Cheng
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Correspondence to Ming-Hsi Huang.

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Huang, CY., Cheng, YJ., Ho, HM. et al. One-pot amphiphilic engineering of bioresorbable polymers for constructing colloidal vesicles and prolonging protein delivery. Polym J 52, 237–244 (2020). https://doi.org/10.1038/s41428-019-0267-3

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  • DOI: https://doi.org/10.1038/s41428-019-0267-3

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