Polymer coating glass to improve the protein antifouling effect


Random copolymers (P(M100-m/T m )) composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), which suppresses protein adsorption, and 3-(triethoxysilyl)propyl methacrylate (MTEOS), which can be covalently fixed on a glass surface, were prepared via photoinitiated radical polymerization. When P(M100-m/T m ) was coated on a glass surface, a protein antifouling effect could be observed because of the presence of MPC units on the glass surface. To confirm the coating of the glass surface with P(M100-m/T m ) by fluorescence microscopy, pyrene-labeled P(M100-m/T m ) was also prepared. An ethanol solution of P(M100-m/T m ) was spin-coated on the glass, which was exposed to NH3 vapor to promote the reaction of the pendant triethoxysilyl groups in P(M100-m/T m ) with silanol groups on the glass. The coating of the glass with MPC was confirmed by fluorescence microscopy. The protein antifouling effects of the P(M100-m/T m )-coated glass were confirmed using fluorescence-labeled proteins. It is expected that P(M100-m/T m ) can be applied as a surface-coating agent on glass containers for protein formulations.

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This work was financially supported by a Grant-in-Aid for Scientific Research (17H03071 and 16K14008) from the Japan Society for the Promotion of Science (JSPS), JSPS Bilateral Joint Research Projects, and the Cooperative Research Program of “Network Joint Research Center for Materials and Devices (20174031).”

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Correspondence to Shin-ichi Yusa.

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Honda, T., Nakao, A., Ishihara, K. et al. Polymer coating glass to improve the protein antifouling effect. Polym J 50, 381–388 (2018). https://doi.org/10.1038/s41428-018-0026-x

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