Fig. 1 | Cellular & Molecular Immunology

Fig. 1

From: Differential retention of lymph-borne CD8 memory T cell subsets in the subcapsular sinus of resting and inflamed lymph nodes

Fig. 1

Distribution of polyclonal memory CD8 T cells in steady-state and MCMV-3D-inflamed popliteal lymph nodes. a Relative distribution of CD8 Tcm and Tem cells in the indicated compartments of steady-state (green symbols) or MCMV-3D-inflamed (blue symbols) popliteal LNs. Dots = mean of 4 sections per 8 PBS-injected and 12 MCMV-3D-inflamed popliteal LNs presented as the mean ± SD. ANOVA; *p < 0.05; **p < 0.01, ****p ≤ 0.0001. b Infiltration of myeloid cells below the SCS of inflamed popliteal LNs. Representative images of steady-state (left) or MCMV-3D-inflamed (right) popliteal LNs collected at 72 h after viral infection (106 PFU MCMV-3D) and 3 h after IL injection of 5 × 104 eFluor450-labeled CD8 Tcm cells (red) and 5 × 104 eFluor670-labeled CD8 Tem cells (green). Counter staining with PE-conjugated anti-CD11b (cyan) and Alexa Fluor 488-conjugated anti-CD11c-biotin/streptavidin (purple) antibodies was performed. The white arrows indicate mCherry+-infected cells, which are visualized in the same channel as anti-CD11b-PE mAbs; scale bars, 100 µm

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