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Structural snapshots of human VMAT2 reveal insights into substrate recognition and proton coupling mechanism

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Fig. 1: The structural basis of substrate recognition and proton coupling of VMAT2.

Data availability

The cryo-EM maps of the human VMAT2P-L, VMAT2NE-L, VMAT2DA-L, VMAT2HA-L, VMAT2MPP-L and VMAT2NE-C have been deposited into the Electron Microscopy Data Bank under accession numbers EMD-38525, EMD-36629, EMD-36639, EMD-36633, EMD-38524 and EMD-38523, respectively. The corresponding coordinates have been deposited into the Protein Data Bank under accession codes 8XOB, 8JSX, 8JTB, 8JT5, 8XOA, 8XO9.

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Acknowledgements

This work is funded by the Chinese Academy of Sciences Strategic Priority Research Program (Grant XDB37030304 to Y.Z.), the National Natural Science Foundation of China (32271272 and T2221001 to D.J., 92157102 to Y.Z.).

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Authors and Affiliations

Authors

Contributions

D.J. and Y.Z. designed the experiments. D.W., Q.C., and N.L. prepared the samples for the cryo-EM study. D.W. and J.Z. collected the cryo-EM data. D.W. and Z.Y. processed the data and built the models. Z.Y., D.W., and Q.C. prepared figures. Q.C. collected the transport activity data. All the authors analyzed and interpreted the results. Y.Z. and D.J. wrote the manuscript, and all the authors reviewed the manuscript.

Corresponding authors

Correspondence to Daohua Jiang or Yan Zhao.

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The authors declare no competing interests.

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Wu, D., Yu, Z., Chen, Q. et al. Structural snapshots of human VMAT2 reveal insights into substrate recognition and proton coupling mechanism. Cell Res (2024). https://doi.org/10.1038/s41422-024-00974-9

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