Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

scNanoCOOL-seq: a long-read single-cell sequencing method for multi-omics profiling within individual cells

Cell Research volume 33, pages 879–882 (2023)Cite this article

Subjects

Dear Editor,

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Overview of scNanoCOOL-seq and its utility in profiling mouse blastocyst cells.

Data availability

All relevant data are available from the Sequence Read Archive database (accession number: PRJNA905717).

Code availability

All codes associated with this study have been deposited on GitHub (https://github.com/sherryxuePKU/scNanoCOOL-seq).

References

  1. Lee, J. et al. Exp. Mol. Med. 52, 1428–1442 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Guo, F. et al. Cell Res. 27, 967–988 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Sakamoto, Y. et al. Nucleic Acids Res. 49, e81 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Zong, C. et al. Science 338, 1622–1626 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Zhou, B. et al. Genome Res. 29, 472–484 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Wang, Y. et al. Nat. Commun. 12, 1247 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Loda, A. et al. Nat. Rev. Mol. Cell Biol. 23, 231–249 (2022).

    Article  CAS  PubMed  Google Scholar 

  8. Sen, M. et al. Nat. Commun. 12, 1286 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Huang, L. et al. Annu. Rev. Genomics Hum. Genet. 16, 79–102 (2015).

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the Basic Science Center Projects of the National Natural Science Foundation of China (32288102), and the Science and Technology Innovation “2030-Brain Science and Brain-like Research” major project (2021ZD0200102).

Author information

Author notes

  1. These authors contributed equally: Jianli Lin, Xiaohui Xue.

Authors and Affiliations

  1. Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China

    Jianli Lin & Fuchou Tang

  2. Biomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing, China

    Jianli Lin, Xiaohui Xue, Yan Wang, Yuan Zhou, Haoling Xie, Mengya Liu, Lu Wen & Fuchou Tang

  3. Beijing Advanced Innovation Center for Genomics (ICG), Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing, China

    Jianli Lin, Xiaohui Xue, Yan Wang, Haoling Xie, Mengya Liu, Lu Wen & Fuchou Tang

  4. PKU-Tsinghua-NIBS Graduate Program, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China

    Xiaohui Xue & Fuchou Tang

  5. Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Lab, Beijing, China

    Jian Wu

Authors
  1. Jianli Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaohui Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuan Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jian Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Haoling Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mengya Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Lu Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Fuchou Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

F.T. conceived and supervised this project. J.L., X.X. and Y.Z. developed protocol. J.L. and Y.Z. performed experiments with help from Y.W. X.X. conducted bioinformatic analysis with assistance from H.X. and M.L. J.L., X.X. and F.T. wrote the manuscript with help from other authors.

Corresponding author

Correspondence to Fuchou Tang.

Ethics declarations

Competing interests

The authors declare no competing interests.

Supplementary information

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lin, J., Xue, X., Wang, Y. et al. scNanoCOOL-seq: a long-read single-cell sequencing method for multi-omics profiling within individual cells. Cell Res 33, 879–882 (2023). https://doi.org/10.1038/s41422-023-00873-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41422-023-00873-5

Search

Advanced search

Quick links