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piRNA 3′ uridylation facilitates the assembly of MIWI/piRNA complex for efficient target regulation in mouse male germ cells

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Fig. 1: piRNA 3′ uridylation promotes piRNA binding to MIWI protein and enhances the targeting capacity of MIWI/piRNA in mouse male germ cells.

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Acknowledgements

We thank members of the M.-F.L. lab and L.-T.G. lab for helpful comments. We thank Drs Marcos Morgan and Dónal O’Carroll from University of Edinburgh for the TAIL-seq datasets of Tut4/7cKO spermatocytes. This work was supported by the National Key R&D Program of China (2017YFA0504400, and 2021YFC2700200), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB19010203), the National Natural Science Foundation of China (91940305, 31830109, 31821004, 31961133022, 91640201, 32170815 and 32101037), Science and Technology Commission of Shanghai Municipality (17JC1420100, 2017SHZDZX01, 19JC1410200, 21PJ1413800, 21ZR1470200, 21YF1452700 and 21ZR1470500), China Postdoctoral Science Foundation (2020T130670 and 2020M671256) and the Foundation of Key Laboratory of Gene Engineering of the Ministry of Education.

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X.W., L.-T.G., and M.-F.L. planned the project. M.-Z.Z., D.-H.L, X.W., L.-T.G., and M.-F.L. designed the experiments. M.-Z.Z., D.-H.L, and X.W. conducted the experiments. H.Z., H.W., and X.W. analyzed the data. X.W., L.-T.G., and M.-F.L. wrote the manuscript.

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Correspondence to Xin Wang, Lan-Tao Gou or Mo-Fang Liu.

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The authors declare no competing interests.

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Zhao, MZ., Lin, DH., Zuo, H. et al. piRNA 3′ uridylation facilitates the assembly of MIWI/piRNA complex for efficient target regulation in mouse male germ cells. Cell Res 32, 1030–1033 (2022). https://doi.org/10.1038/s41422-022-00659-1

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