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Molecular basis of ligand recognition and activation of human V2 vasopressin receptor

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Fig. 1: Cryo-EM structure of the AVP–V2R–Gs complex.

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Acknowledgements

We thank all staff members of the Cryo-EM Centre, Southern University of Science and Technology for their assistance in data collection. This work was partially supported by the Ministry of Science and Technology (China) grant (2018YFA0507002 to H.E.X.); National Natural Science Foundation of China (31770796 to Y.J., 81872915 to M,-W.W., 31600606 to X.Z., and 81773792 to D.Y.); National Science & Technology Major Project “Key New Drug Creation and Manufacturing Program” (2018ZX09711002-002-002 to Y.J., 2018ZX09735-001 to M.-W.W., and 2018ZX09711002-002-005 to D.Y.); Shanghai Municipal Science and Technology Major Project (2019SHZDZX02 to H.E.X.); CAS Strategic Priority Research Program (XDB37030103 to H.E.X.); Start-up funding by Fudan University (Q.Z.); Wellcome Trust 209407/Z/17/Z; National Key R&D Program of China (2016YFA0501100 to X.Z.); Guangdong Provincial Key Laboratory of Brain Connectome and Behavior (2017B030301017 to X.Z.); CAS Key Laboratory of Brain Connectome and Manipulation (2019DP173024 to X.Z.).

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F.Z. prepared samples for cryo-EM grid preparation; F.Z. and X.M. performed cryo-EM data collection and processing; F.Z., T.C., X.M., and X.Z. built and refined the structure model; C.Y. and D.Y. conducted functional studies and data analysis; Q.Z. and X.H. carried out docking analysis; W.Y. engineered the mini-Gs protein; F.Z. and Y.J. prepared the bulk of figures, performed the structural analysis, and drafted the manuscript; Y.J., H.E.X., and M.-W.W. supervised the studies, analyzed the data, and wrote the manuscript with inputs from all co-authors; P.W. supervised the EM studies.

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Correspondence to Peiyi Wang or H. Eric Xu or Ming-Wei Wang or Yi Jiang.

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The authors declare no competing interests.

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Zhou, F., Ye, C., Ma, X. et al. Molecular basis of ligand recognition and activation of human V2 vasopressin receptor. Cell Res (2021). https://doi.org/10.1038/s41422-021-00480-2

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