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Mitochondrial replacement in macaque monkey offspring by first polar body transfer

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Fig. 1: Generation of live monkey offspring using oocytes reconstructed by PB1T.


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We thank Professor Muming Poo for his critical discussion and suggestion, and Lina Wang, Jia Liu, Jiantao Huang, Changshan Gao for help with animal breeding and care. This work was supported by the National Key R&D Program of China (2018YFC1003000 to Q.S. and Z.L.), the National Natural Science Foundation of China (31825018 to Q.S.), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB32060100 to Q.S.), the Shanghai Municipal Science and Technology Major Project (2018SHZDZX05 to Q.S.), the Shanghai Municipal Government Bureau of Science and Technology (18JC1410100 to Q.S.), and Shanghai Young Talents Sailing Program (18YF1427500 to Z.L.).

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Z.L. and Q.S. designed and supervised the project. Z.L. performed the embryo manipulation experiments. Z.L. and Z.W. performed mtDNA copy number, immunostaining, mitochondrial distribution, mtDNA carryover experiments. Y.W., Y.N., X.Z., and Y.X. performed monkey assisted reproductive experiments. Y.W. and Q.L. performed monkey physical examination. X.Y. and Z.W. performed single-cell DNA methylation analysis. Z.W. performed single-cell whole-genome sequencing analysis. Z.L., Z.W., and Q.S. prepared the manuscript with contributions from all other authors.

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Correspondence to Zhen Liu or Qiang Sun.

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The authors declare no competing interests.

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Wang, Z., Li, Y., Yang, X. et al. Mitochondrial replacement in macaque monkey offspring by first polar body transfer. Cell Res 31, 233–236 (2021).

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