Fig. 1: Establishment of intestinal organoid cultures enriched for an injury-associated regenerative signature. | Cell Research

Fig. 1: Establishment of intestinal organoid cultures enriched for an injury-associated regenerative signature.

From: Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration

Fig. 1

a qPCR analyses of Clu and regenerative gene expression in organoids cultured under the indicated conditions (n = 2 wells). P values were determined by two-sided unpaired t-test. b Typical morphology of intestinal organoids cultured under the indicated conditions. c Immunofluorescence staining of CLU and regenerative markers in organoids cultured under the indicated conditions. d FACS analysis of SCA1+ cells in organoids cultured under the indicated conditions (n = 3 mice). P values were determined by two-sided unpaired t-test. e Quantification of CLU+ cells in each organoid cultured under the indicated conditions (n = 15 organoids from three mice). P values were determined by two-sided unpaired t-test. f Heatmap displaying the expression of an injury-associated regenerative signature in different organoids and primary crypts from different intestinal injury models (n = 3 mice). The gene expression profile of organoids cultured in the ENR and 8C conditions was plotted. Representative genes are shown on the left. Gran and Non-gran indicate crypts overlying and adjacent to granulomas, respectively. DSS and Non-DSS indicate crypts from repairing epithelium in DSS-associated colitis, and normal epithelium without DSS treatment, respectively. **P < 0.01; ***P < 0.001. Scale bars, 100 μm. The experiments in ac were independently repeated at least three times with similar results.

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