METTL4 is an snRNA m6Am methyltransferase that regulates RNA splicing

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: METTL4 is an internal m6Am methyltransferase regulating RNA splicing.

References

  1. 1.

    Boccaletto, P. et al. Nucleic Acids Res. 46, D303–D307 (2018).

  2. 2.

    Shi, H., Wei, J. & He, C. Mol. Cell 74, 640–650 (2019).

  3. 3.

    Akichika, S. et al. Science 363, eaav0080 (2019).

  4. 4.

    Boulias, K. et al. Mol. Cell 75, 631–643 e638 (2019).

  5. 5.

    Sendinc, E. et al. Mol. Cell 75, 620–630 e629 (2019).

  6. 6.

    Sun, H., Zhang, M., Li, K., Bai, D. & Yi, C. Cell Res. 29, 80–82 (2019).

  7. 7.

    Mauer, J. et al. Nat. Chem. Biol. 15, 340–347 (2019).

  8. 8.

    Bujnicki, J. M., Feder, M., Radlinska, M. & Blumenthal, R. M. J. Mol. Evol. 55, 431–444 (2002).

  9. 9.

    Ma, C. et al. Nat. Cell Biol. 21, 319–327 (2019).

  10. 10.

    Kweon, S. M. et al. Mol. Cell 74, 1138–1147 e1136 (2019).

  11. 11.

    Greer, E. L. et al. Cell 161, 868–878 (2015).

  12. 12.

    Wei, J. et al. Mol. Cell 71, 973–985 e975 (2018).

  13. 13.

    Reddy, R., Henning, D., Epstein, P. & Busch, H. Nucleic Acids Res. 9, 5645–5658 (1981).

  14. 14.

    Lee, Y. & Rio, D. C. Annu. Rev. Biochem. 84, 291–323 (2015).

Download references

Acknowledgements

We thank Chunmei Li, Matthew Harris, Erdem Sendinc, and David Valle-Garcia for insightful advice. Y.S. is supported by BCH funds. R.I.G. is a recipient of the NCI Outstanding Investigator Award (R35 CA232115-01). E.A.O. was supported by the Pew Latin American Fellows Program and by a Damon Runyon Fellowship (DRG-2378-19). Y.X. is supported by NIH grants (R01GM088342 and R01GM121827). Y.S. is an American Cancer Society Research Professor.

Author information

Y.S. and H.C. conceived and designed the project. H.C., L.G., J.G. and Q.L. carried out most of the biochemical and cellular experiments. Y.W. performed the bioinformatic analysis under supervision of Y.X. E.A.O. performed m6A Northwestern analysis under supervision of R.I.G. L.W. and Z.S. contributed to characterization of enzymatic activity under supervision of H.W. Y.S. supervised the project throughout. Y.S. and H.C. co-wrote the manuscript and all authors contributed to manuscript writing.

Correspondence to Yang Shi.

Ethics declarations

Competing interests

R.I.G. is a co-founder, scientific advisory board member, and equity holder of 28-7 Therapeutics. Y.X. is a scientific cofounder of Panorama Medicine. Y.S. is a co-founder and equity holder of Constellation Pharmaceuticals, Inc., a consultant of Guangzhou BeBetter Medicine Technology Co., LTD and an equity holder of Imago Biosciences. All other authors declare no competing financial interests.

Supplementary information

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Chen, H., Gu, L., Orellana, E.A. et al. METTL4 is an snRNA m6Am methyltransferase that regulates RNA splicing. Cell Res (2020). https://doi.org/10.1038/s41422-019-0270-4

Download citation