Structural insights into Cas13b-guided CRISPR RNA maturation and recognition

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Acknowledgements

This work was supported by the Ministry of Science and Technology of China grants 2014CB910400 and the National Nature Science Foundation of China grants 31770948, 31570875 and 81590761. We thank Fujian Normal University for financial support. The diffraction data were collected at the beamline BL-17U1 of Shanghai Synchrotron Radiation Facility (SSRF). We thank Gonghong Wei and Zhaoqing Luo for discussions. We thank Wei Yang and Zhijian J. Chen for critical review of the manuscript.

Author information

B.Z. and W.Y. purified the BzCas13b-crRNA complex and the BzCas13b mutants. B.Z and Y.Y. purified the in vitro transcribed RNAs and performed the cleavage assays. B.Z., W.Y. and Y.Y. performed the crystallization screening. B.Z. optimized the crystallization condition. H.Z. and B.Z. collected X-ray diffraction data. B.Z. solved the crystal structure. J.C., J.L. and V.P. contributed to some experiments. B.Z., S.O., Q.C., C.C., M.C. and M.I.C. designed the experiments. S.O. and B.Z. supervised the study. B.Z., A.S., S.O. wrote the manuscript.

Correspondence to Songying Ouyang.

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Supplementary information, Data S1

Supplementary information, Movie S1

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