Reversible regulation of Cas12a activities by AcrVA5-mediated acetylation and CobB-mediated deacetylation

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associated (Cas) system protects bacteria and archaea from the invasion of foreign genetic elements. To cope with the host CRISPR systems, phages have evolved many strategies, including the anti-CRISPR (Acr) proteins, to inactivate the Cas nucleases. Recently, it has been reported that the type V-A Cas12a effector can be acetylated and inactivated by AcrVA5, which is a GNAT-family acetyltransferase. However, it is unclear whether the host has any coping strategies to reactivate the defense system. Here we show that the AcrVA5-acetylated Cas12a can be deacetylated by bacterial deacetylase CobB, reactivating Cas12a for both in vitro cleavage of target DNA sequences and in vivo protection of the host from invasion of foreign nucleic acids. Therefore, this study not only shows the reversible regulation of Cas12a activities by post-translational modification but also reveals CobB as a secondary safeguard to bacterial CRISPR defense systems. In addition, we demonstrate that AcrVA5 is a wide-spectrum acetyltransferase, acetylating a large number of target proteins besides Cas12a, and the AcrVA5-acetylated targets can also be deacetylated by CobB.


C O R R E S P O N D E N C E O p e n A c c e s s
Reversible regulation of Cas12a activities by AcrVA5-mediated acetylation and CobB-mediated deacetylation Xiaoman Kang 1,2 , Lei Yin 3 , Songkuan Zhuang 4 , Tianshuai Hu 4 , Zhile Wu 5 , Guoping Zhao 1 , Yijian Chen 6,7 ✉ , Yong Xu 4✉ and Jin Wang 4,8 ✉ Dear Editor, The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associated (Cas) system protects bacteria and archaea from mobile genetic elements (MGEs) such as bacteriophages 1 . The Cas effector can be guided by a CRISPR RNA (crRNA) to target the invading nucleic acids by base-pairing and then cleaves the target to provide the host with immunity 2,3 . To cope with the immunity pressure imposed by the host CRISPR system, phages have evolved different kinds of anti-CRISPR (Acr) systems to inactivate the Cas nucleases and close the host immunity system in turn 4 . The Acr proteins can directly interact with the Cas proteins to prevent the crRNA loading or target binding; alternatively, they possess enzymatic activities to cleave the crRNAs or post-translationally modify the Cas proteins 4 . Among them, there exists a recently reported GNAT-family acetyltransferase, namely AcrVA5, which is encoded by the MGEs and specifically inhibits the type V-A Cas12a effector through acetyl modification of a key lysine site. The AcrVA5-inactivated Cas12a loses the ability to interact with the protospacer adjacent motif (PAM) site and fails in protecting the hosts by cleaving the invading MGEs 5,6 . Through inactivating the Cas12a system, MGEs containing the acrVA5 gene can effectively shut down the bacterial immunity system and have the potential to widely spread among the Cas12a-harboring hosts. However, to our great surprise, the acrVA5 gene exists only in three Moraxella bovoculi strains which have lost their deacetylases (Supplementary Table S1). Therefore, it is reasonable to suspect that there may exist a competitive relationship between the acetyltransferase AcrVA5 and the widely distributed bacterial deacetylases 7 , which may reactivate Cas12a by deacetylation to protect the hosts from the invasion of MGEs harboring the acrVA5 cassette.
We first performed the AcrVA5-mediated in vitro acetylation experiment and showed that the AcrVA5-acetylated Lachnospiraceae bacterium (Lb) Cas12a lost both cisand trans-cleavage activities towards double-stranded target DNA (dsDNA) ( Supplementary Fig. S1a, b), which was consistent with the previous findings 5, 6 , however, the AcrVA5-treatment showed no effect on LbCas12a transcleavage activities when triggered by target singlestranded DNA (ssDNA) ( Supplementary Fig. S1c). As the PAM site is only necessary for Cas12a to recognize target dsDNA but not ssDNA 8 , the above results further confirmed that AcrVA5-mediated acetylation prevented Cas12a from interacting with the PAM sequences in target dsDNA 5 .
Besides LbCas12a, we also analyzed Cas12a orthologs from Francisella tularensis subsp. novicida (FnCas12a) and Acidaminococcus sp. (AsCas12a) and demonstrated that AcrVA5 was able to inactivate both orthologs (Supplementary Figs. S2 and S3). Noticeably, AcrVA5 was ever shown to be ineffective against AsCas12a in a previous study 6 , however, we found that AsCas12a contained the conserved lysine residue ( Supplementary Fig. S3c) and showed that the AcrVA5-mediated treatment led to the loss of both cisand trans-cleavage activities of AsCas12a with target dsDNA.
Post-translational lysine acetylation plays an important role in diverse cellular processes in organisms from bacteria to human 9 . In bacteria, the NAD + -dependent sirtuin-type CobB deacetylates a large number of proteins and regulates the global acetylation level 7 . To test whether CobB can deacetylate the AcrVA5-treated Cas12a and reactivate its cisand trans-cleavage activities, we then purified recombinant E. coli CobB and LbCas12a and performed the in vitro deacetylation assay. Based on the western blot results, Cas12a was successfully acetylated by AcrVA5 at the presence of acetyl-CoA, and the acetyl modification could be efficiently removed after being treated by CobB. Consistent with previous findings 7 , the CobB-mediated deacetylation stringently requires NAD + as the cofactor (Supplementary Fig. S4). Accordingly, the AcrVA5-acetylated LbCas12a lost both cisand transcleavage activities with target dsDNA but recovered both activities to a large extent after being deacetylated by CobB (Fig. 1a, b). In addition, we tested several Cas12a orthologs such as FnCas12a and AsCas12a and found CobB was able to deacetylate and reactivate both Cas12a orthologs (Supplementary Figs. S5-S8). It was worthy to mention that the successful reactivation of acetylated AsCas12a by CobB treatment once again proved AsCas12a as a target of AcrVA5 ( Supplementary  Fig. S3), while the reason for the distinct results between this work and the previous study 6 was still unknown and could be an interesting question subject to further investigation. Based on the above results, one may conclude that AcrVA5 and CobB reversibly regulate both the cisand trans-cleavage activities of Cas12a by modulating its acetyl status in vitro.
We further explored the physiological role of CobB in protecting the Cas12a-containing host from the invasion of MGEs that harbor the acrVA5 gene. We first constructed a CRISPR plasmid that expressed both LbCas12a and a crRNA targeting a specific sequence in the apramycin resistance gene (apr) and transformed it into E. coli strains with or without a high level of CobB expression (Supplementary Fig. S9). Because of the low expression level of the native cobB gene in E. coli 10 ( Supplementary Fig. S10), the wild type W3110 as well as the cobB-deleted strains were considered as the cobB-negative host, while the cobB overexpression strain was the cobB-positive host. To prepare a reporter plasmid without the targeting sequence of the Cas12a/crRNA complex, we mutated the Cas12atargeting DNA sequence in the apr gene without changing the amino acid sequence as well as the apramycin resistance ( Supplementary Fig. S11), obtaining a mutated apr gene. Because the nonsense mutation changed both the PAM site and the guide sequence, Cas12a failed to cleave the target sequence both in vitro (Supplementary Fig.  S11b). As a result, the reporter plasmid with mutant apr effectively escaped from the host defense system and showed high transformation efficiencies in an acrVA5independent manner (Fig. 1c), which was therefore employed as a control of the transformation assay.
The reporter plasmid containing the wild type apr gene was then transformed into E. coli strains expressing Cas12a to mimic the invasion of MGEs, and as expected, in the absence of acrVA5 gene, no transformants could be obtained in all tested strains independent on the expressional level of CobB, representing the invasion of the foreign plasmid could be completely blocked by the hosts (Fig. 1c). However, if the reporter plasmid contained both apr and acrVA5, mimicking MGEs harboring the acrVA5 cassette, the high transformation efficiency of the reporter plasmid was obtained in the cobB-negative hosts, which was consistent with the above findings that AcrVA5 effectively inactivated Cas12a. As the overexpression of CobB in the cobB-positive host could deacetylate and reactivate the AcrVA5-acetylated Cas12a, the transformation of the reporter plasmid was largely blocked. As expected, the efficiency of the reporter plasmid was still much lower than the control, although overexpression of CobB and AcrVA5 may severely reduce the E. coli transformation efficiencies (Fig. 1c). Based on the above results, one may conclude that AcrVA5 and CobB reversibly regulated the Cas12a activities in vivo through acetylation and deacetylation, respectively.
As the AcrVA5-mediated acetyl modification of Cas12a has been considered as an effective anti-Cas12a strategy by MGEs 5,6 , the CobB-mediated deacetylation reactivation of Cas12a through modification can be taken as both a prevention strategy to the anti-CRISPR elements and a safeguard to the CRISPR defense system, protecting hosts from the invading of the acrVA5-containing MGEs (Fig. 1d). Moreover, this study highlights the potential to create more complexed systems to regulate the Cas12a cleavage activities, which may facilitate both in vivo gene editing and in vitro target nucleic acid detection 11 .
Besides Cas12a, AcrVA5 may function as a broadspectrum acetyltransferase and influences cellular metabolic processes. We next performed the in vitro western blot analysis through using E. coli cellular extracts and purified AcrVA5 and found a large number of proteins could be acetylated by AcrVA5 (Supplementary Fig. S12). Similarly, after overexpression of AcrVA5 in E. coli, the whole cellular acetylation level was greatly increased, and the acetylated proteins could then be efficiently deacetylated by CobB at the presence of NAD + (Supplementary Fig.  S13). With the employment of mass spectrometry, we further characterized 2688 sites and 1101 proteins as potential AcrVA5 targets (Supplementary Table S2). Probably due to the small protein size and the reduced steric hindrance in turn, AcrVA5 showed no obvious target sequence preference but favored positively charged amino acids such as lysine, arginine, and histidine ( Supplementary Fig. S14). Considering the extensive targets of AcrVA5, one may imagine that CobB and AcrVA5 compete for control of both the host immune system and beyond. Moreover, the Dsr proteins, which contain the deacetylase domains, were recently found to protect hosts against the invading of dsDNA phages 12 , and the present study may possibly provide a clue for the unclear mechanisms.
Taken together, one could infer that the wars between invading MGEs and the microbial hosts will never end, and besides acetyl-and deacetyl-modification, there probably exist other kinds of competitive mechanisms subject to future investigation. While just as the Chinese idiom goes, one can believe a vice rises one foot, but virtues rise ten.