a Schematics of the
experimental design for single-cell RNA (sc-RNA) sequencing.
Peripheral blood mononuclear cells (PBMCs) were collected from
COVID-19 patients and healthy controls (HCs) and then processed
via sc-RNA, sc-BCR, and sc-TCR sequencing using the 10x-Based
Genomics platform. b The
heatmaps show differentially expressed genes (DEGs) upregulated
in myeloid cells, NK and T cells, B cells, and other clusters of
PBMCs. c t-distributed
stochastic neighbor embedding (t-SNE) plot showing myeloid cells
(red), NK and T cells (blue), B cells (green), and other
clusters (gray) of PBMCs identified using integrated and
classification analysis. d
t-SNE projection of canonical markers, including CD14, CD1C, and FCGR3A for myeloid cells; CD3E, CD4, CD8A, and NCAM1 for NK and T cells; andCD19 for B cells as
indicated in the legend.