Fig. 3: DDIT3 is a key mediator of SOX4 repression and Wnt signaling inhibition. | Cell Discovery

Fig. 3: DDIT3 is a key mediator of SOX4 repression and Wnt signaling inhibition.

From: Cancer cell specific inhibition of Wnt/β-catenin signaling by forced intracellular acidification

Fig. 3

ae Levels of the indicated mRNA and proteins in the indicated cell lines were monitored by qRT-PCT or WB, respectively. a SOX4 rescue by DDIT3 loss-of-function. Error bars represent mean values ± SD. b SOX4 rescue in cells with DDIT3 knockout (DDIT3-/-(CRISPR)) in comparison to wildtype. Metformin treatment  -  35 h. c SOX4 inhibition caused by overexpression of DDIT3 in DDIT3TET-ON cells induced by Doxycycline (Dox). d DDIT3 induction and removal of SOX4 protein caused by ER drugs, Tunicamycin and Bortezomib. e SOX4 rescue caused by siRNA-mediated knockdown of the indicated members of the UPR.

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