Fig. 2: Silencing of lncRNA ZNF674-AS1 significantly inhibited the proliferation of GCs. | Cell Death Discovery

Fig. 2: Silencing of lncRNA ZNF674-AS1 significantly inhibited the proliferation of GCs.

From: LncRNA ZNF674-AS1 regulates granulosa cell glycolysis and proliferation by interacting with ALDOA

Fig. 2

A, B The viability of KGN and COV434 cells after ZNF674-AS1 silencing was assessed by CCK8 assay. Results are expressed as the mean ± SD (n = 3). ***p < 0.001 by two-tailed Student’s t test. C, D The proliferation of KGN cells after ZNF674-AS1 silencing was evaluated by EdU staining assay. Results are expressed as the mean ± SD (n = 3). ***p < 0.001 by two-tailed Student’s t test. Scale bar: 200 μm. E, F The proliferation of COV434 cells after ZNF674-AS1 silencing was evaluated by EdU staining assay. Results are expressed as the mean ± SD (n = 3). **p < 0.01 by two-tailed Student’s t test. Scale bar: 50 μm. G, H PCNA protein levels in KGN and COV434 cells were analyzed by western blot after ZNF674-AS1 silencing. For gray value quantification of PCNA, data were normalized to the internal reference. Shown are mean ± SD. *p < 0.05, ***p < 0.001 by two-tailed Student’s t test.

Back to article page