Fig. 6: ERK5 pharmacological inhibition blocks NF-kB/IL-8 signaling. | Cell Death Discovery

Fig. 6: ERK5 pharmacological inhibition blocks NF-kB/IL-8 signaling.

From: MEK5/ERK5 activation regulates colon cancer stem-like cell properties

Fig. 6

a HCT116 cells were cultured for 7 days under sphere-forming conditions in the presence of 4 µM XMD8-92 or dimethyl sulfoxide (DMSO) vehicle control. IκB phosphorylation levels were evaluated by western blot. b Nuclear factor-κB (NF-κB) transcriptional activity was assayed using an inducible luciferase reporter system. c, d HCT116 cells were transfected with NF-κB p65 or DN-IκBα expression constructs, and empty vector, and then treated for 48 h with 4 µM XMD8-92. DMSO was used as vehicle control. c NF-κB and IκBα overexpression was confirmed by immunoblotting. d IL-8 mRNA levels were determined by qRT-PCR. Results are expressed as mean ± standard error of mean from three independent experiments. *p < 0.05 and **p < 0.01 from vehicle control treatment; p < 0.01 from empty vector cells. DN dominant negative

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