Fig. 6: C3 D9E is inefficiently cleaved resulting in decreased caspase activation and cell death. | Cell Death Discovery

Fig. 6: C3 D9E is inefficiently cleaved resulting in decreased caspase activation and cell death.

From: The prodomain of caspase-3 regulates its own removal and caspase activation

Fig. 6

a Expression of cells stably expressing C3 and C3 D9E. b A caspase-3 activity assay was used to determine the ability to cleave a DEVD-chromphore substrate. c Cell death was determined using Annexin V/PI staining and flow cytometry. d Cells were serum starved for 24 h and the cleavage pattern of caspase-3 was determined using western blot analysis. Actin was used as a loading control. Data are presented as mean ± SEM of at least three independent experiments. *p > 0.05, **p > 0.01, ***p > 0.001

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