Table 3 Ranking of genes in positive selection tunicamycin screen using MAGeCK

From: SEC24A identified as an essential mediator of thapsigargin-induced cell death in a genome-wide CRISPR/Cas9 screen

Rank Gene Number of sgRNAsa RRA scoreb p c FDR Good sgRNAs LFCd
1 MFSD2A 6 6.27E−26 2.27E−07 0.00495 6 11.262
2 TBX5 6 8.40E−05 0.00016734 0.905049 2 −5.0824
3 AGPHD1 3 0.00021431 0.00023182 0.905049 1 −6.6886
4 C1orf95 5 0.00027313 0.00032083 0.905049 1 −6.3316
5 ALDH4A1 6 0.00027733 0.00036397 0.905049 1 −6.3373
6 TMED7-TICA 5 0.00027733 0.00045343 0.905049 1 −7.1405
7 FBXO42 6 0.0005294 0.00061282 0.905049 1 −7.097
8 UROS 6 0.0005798 0.00066323 0.905049 1 −7.2471
9 CHURC1 6 0.0006302 0.00070501 0.905049 1 −7.4473
10 APOBEC1 6 0.00068061 0.00075224 0.905049 1 −6.9459
  1. FDR false discovery rate
  2. aNumber of sgRNAs refers to number of sgRNAs targeting the gene
  3. bRRA score is derived from a robust ranking aggregation (RRA) algorithm that determines the degree of consistency seen in the enrichment of multiple sgRNAs targeting a particular gene, compared to sgRNAs targeting other genes
  4. cp Value is generated for these RRA scores
  5. dLFC is log fold change of sgRNAs