Fig. 7: Identification of essential composition of AU that was responsible for the improvement of cell polarization. | Cell Death Discovery

Fig. 7: Identification of essential composition of AU that was responsible for the improvement of cell polarization.

From: More complete polarization of renal tubular epithelial cells by artificial urine

Fig. 7

Each composition of the AU was evaluated, one at a time, for its effect on ΔTER as calculated from subtraction of the TER at 84-h post-culture (after switching culture medium in the upper chamber for 24 h) from that measured at 60-h post-culture (before switching culture medium in the upper chamber) (a). ‘AU – X’ represents the absence of X in the AU formula. To confirm the essential role of urea, the ΔTER was again evaluated by using 200 mM urea when switching the culture medium in the upper chamber, comparing to serum-containing medium (control), standardized AU (pH 6.2; containing 200 mM urea), and ‘AU – Urea’ (AU without any urea) (b). Each bar represents mean ± SEM of the data obtained from three independent biological replicates. *** p < 0.001 vs. control; ## p < 0.01 vs. AU (pH 6.2); ### p < 0.001 vs. AU (pH 6.2)

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