Fig. 6: Inactivation of Lon protease results in the accumulation of unfolded mitochondrial proteins. | Cell Death Discovery

Fig. 6: Inactivation of Lon protease results in the accumulation of unfolded mitochondrial proteins.

From: Lon protease inactivation in Drosophila causes unfolded protein stress and inhibition of mitochondrial translation

Fig. 6

a Triton-insoluble mitochondrial proteins detected by western blot in heads from 1-day-old control and LonKD flies, using antibodies to complex Vβ, PDHα, aconitase, and NDUFS3. The results were quantified by ratio to actin and normalized to control levels. The experiment was repeated at least three times. *p< 0.05, **p< 0.005, ***p< 0.0005 by Student’s t-test. b Immunoblot analysis of head proteins using antibodies to Hsp60A and Hsc70-5. Protein was extracted from heads of day 1 control and LonKD flies using RIPA buffer. Quantification was performed as in (a). Experiments were repeated at least three times. *p< 0.05, **p< 0.005 by Student’s t-test. Control = UAS-mCherry-RNAi driven by elav-GAL4 and da-GAL4. LonKD = UAS-Lon-RNAi-1 driven by elav-GAL4 and da-GAL4

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