Fig. 5: Mitochondrial RNAs accumulate in large untranslated particles upon Lon knockdown. | Cell Death Discovery

Fig. 5: Mitochondrial RNAs accumulate in large untranslated particles upon Lon knockdown.

From: Lon protease inactivation in Drosophila causes unfolded protein stress and inhibition of mitochondrial translation

Fig. 5

qRT-PCR analysis of individual sucrose gradient fractions was used to characterize the distribution of the indicated mtDNA-encoded mRNAs relative to the 28S and 39S subunits, and fully assembled 55S mitochondrial ribosomes. Mitochondrial homogenates for sucrose density fractionation were prepared from 21-day-old adult LonKD and control flies. Relative abundance represents the fraction of the mRNA in any given fraction relative to the total after normalizing to a luciferase control RNA that was added to each of the fractions prior to RNA isolation. Control = UAS-mCherry-RNAi driven by elav-GAL4 and da-GAL4. LonKD = UAS-Lon-RNAi-1 driven by elav-GAL4 and da-GAL4

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