Fig. 4: Mitochondrial ribosome assembly is only mildly affected in LonKD flies. | Cell Death Discovery

Fig. 4: Mitochondrial ribosome assembly is only mildly affected in LonKD flies.

From: Lon protease inactivation in Drosophila causes unfolded protein stress and inhibition of mitochondrial translation

Fig. 4

Mitochondrial lysates from 21-day-old control and LonKD flies were subjected to sucrose density gradient fractionation to assess the state of assembly of mitochondrial ribosomes. The relative proportions of the small (28S) subunit, large (39S) subunit, and fully assembled (55S) mitochondrial ribosomes were assessed by subjecting the density gradient fractions to qRT-PCR using primer sets specific to 12S rRNA, which marks the small subunit, and to 16S rRNA, which marks the large subunit. Co-localization of the 12S and 16S rRNAs is diagnostic of fully assembled and actively translating ribosomes. Fractions containing the small (28S) subunit, large (39S) subunit, and fully assembled (55S) ribosome are shaded cyan, pink, and yellow, respectively. The relative abundance of a given rRNA in each fraction was calculated as the percentage relative to the total RNA abundance in all fractions after normalizing to a luciferase control RNA that was spiked into each of the fractions prior to RNA isolation. Control = UAS-mCherry-RNAi driven by elav-GAL4 and da-GAL4. LonKD = UAS-Lon-RNAi-1 driven by elav-GAL4 and da-GAL4

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